Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3530763..3531433 Replicon chromosome
Accession NZ_CP007803
Organism Mycobacterium tuberculosis K

Toxin (Protein)


Gene name higB Uniprot ID O53332
Locus tag MTBK_RS16665 Protein ID WP_003899954.1
Coordinates 3530763..3531107 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0THF6
Locus tag MTBK_RS16670 Protein ID WP_003899955.1
Coordinates 3531104..3531433 (+) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MTBK_RS16635 3525836..3526696 + 861 WP_003416628.1 alpha/beta hydrolase -
MTBK_RS16640 3526791..3527186 + 396 WP_071854247.1 nitroreductase family deazaflavin-dependent oxidoreductase -
MTBK_RS16645 3527426..3527719 + 294 WP_003416635.1 hypothetical protein -
MTBK_RS16650 3528007..3529296 + 1290 WP_003416640.1 ATP-binding protein -
MTBK_RS16655 3529643..3530077 - 435 WP_003899952.1 type II toxin-antitoxin system VapC family toxin -
MTBK_RS16660 3530080..3530532 - 453 WP_003899953.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
MTBK_RS16665 3530763..3531107 + 345 WP_003899954.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
MTBK_RS16670 3531104..3531433 + 330 WP_003899955.1 XRE family transcriptional regulator Antitoxin
MTBK_RS16675 3531971..3532318 + 348 WP_003899956.1 DUF2384 domain-containing protein -
MTBK_RS16680 3532315..3532935 + 621 WP_003899957.1 RES family NAD+ phosphorylase -
MTBK_RS16685 3533095..3534360 - 1266 WP_003902423.1 hypothetical protein -
MTBK_RS16690 3534528..3534737 + 210 WP_003416778.1 hypothetical protein -
MTBK_RS16700 3534984..3536018 - 1035 WP_003416786.1 IS30 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-91)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12692.50 Da        Isoelectric Point: 5.6920

>T47140 WP_003899954.1 NZ_CP007803:3530763-3531107 [Mycobacterium tuberculosis K]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Download         Length: 345 bp

>T47140 NZ_CP007803:3530763-3531107 [Mycobacterium tuberculosis K]
TTGGCCGTGATCCTGCTCCCGCAGGTCGAACGGTGGTTCTTCGCGCTCAACAGGGATGCGATGGCCTCGGTCACCGGCGC
CATCGACCTGCTCGAAATGGAGGGGCCGACGTTGGGCCGCCCGGTGGTCGACAAAGTGAACGACTCAACGTTTCACAACA
TGAAGGAGCTGCGCCCCGCCGGCACCAGCATCCGGATCCTGTTCGCCTTCGACCCGGCCCGGCAGGCGATCCTGCTGCTG
GGCGGTGACAAGGCAGGCAACTGGAAACGCTGGTACGACAACAACATTCCAATCGCTGACCAGCGCTCCGAGAACTGGCT
GGCGAGCGAGCACGGAGGTGGATGA

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 11802.46 Da        Isoelectric Point: 7.4051

>AT47140 WP_003899955.1 NZ_CP007803:3531104-3531433 [Mycobacterium tuberculosis K]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Download         Length: 330 bp

>AT47140 NZ_CP007803:3531104-3531433 [Mycobacterium tuberculosis K]
ATGACCATGGCCCGCAACTGGCGTGACATTCGCGCCGATGCCGTCGCGCAGGGCCGCGTGGATCTGCAGCGGGCGGCCGT
GGCACGCGAGGAGATGCGCGATGCCGTCCTGGCGCACCGCCTGGCCGAGATCCGCAAGGCGCTAGGCCACGCACGTCAGG
CCGACGTCGCGGCGCTGATGGGGGTCTCTCAGGCCCGTGTCTCCAAGCTGGAGAGCGGCGACCTGTCCCACACCGAACTC
GGCACCCTGCAGGCCTACGTTGCCGCCCTGGGCGGGCACCTGCGCATCGTCGCTGAGTTCGGCGAAAATACTGTCGAGCT
GACCGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBK2

Antitoxin

Source ID Structure
PDB 6LTY
PDB 6LTZ
AlphaFold DB G0THF6

References