47116

Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) higBA (relBE)/Gp49-HTH_37
Location 2174264..2175132 Replicon chromosome
Accession NZ_CP007803
Organism Mycobacterium tuberculosis K

Toxin (Protein)


Gene name higB Uniprot ID P9WJA4
Locus tag MTBK_RS10095 Protein ID WP_010886136.1
Coordinates 2174264..2174641 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0TLM0
Locus tag MTBK_RS10100 Protein ID WP_003409886.1
Coordinates 2174683..2175132 (+) Length 150 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MTBK_RS10045 2170053..2170505 - 453 WP_003899095.1 lipoprotein -
MTBK_RS10050 2170569..2170970 + 402 WP_003409869.1 hypothetical protein -
MTBK_RS10055 2170963..2171145 - 183 WP_019250140.1 hypothetical protein -
MTBK_RS10060 2171259..2171609 - 351 WP_003409871.1 hypothetical protein -
MTBK_RS10065 2171620..2172522 - 903 WP_003899097.1 hypothetical protein -
MTBK_RS10070 2172543..2172734 - 192 WP_003409876.1 hypothetical protein -
MTBK_RS10075 2172735..2173031 - 297 WP_003409877.1 hypothetical protein -
MTBK_RS10080 2173271..2173486 + 216 WP_003409878.1 antitoxin -
MTBK_RS10085 2173483..2173794 + 312 WP_003409881.1 type II toxin-antitoxin system VapC family toxin -
MTBK_RS22420 2173768..2174289 - 522 WP_003904745.1 hypothetical protein -
MTBK_RS10095 2174264..2174641 + 378 WP_010886136.1 type II toxin-antitoxin system toxin HigB Toxin
MTBK_RS10100 2174683..2175132 + 450 WP_003409886.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
MTBK_RS10105 2175129..2175674 + 546 WP_003409891.1 SecB-like chaperone -
MTBK_RS10110 2175563..2176177 - 615 WP_003901296.1 hypothetical protein -
MTBK_RS10115 2176226..2176522 - 297 WP_003409896.1 type II toxin-antitoxin system RelE/ParE family toxin -
MTBK_RS10120 2176519..2176770 - 252 WP_003409899.1 type II toxin-antitoxin system ParD family antitoxin -
MTBK_RS10125 2176757..2177251 + 495 WP_003899099.1 hypothetical protein -
MTBK_RS10130 2177411..2177818 - 408 WP_003409913.1 type II toxin-antitoxin system VapC family toxin -
MTBK_RS10135 2177822..2178094 - 273 WP_003899100.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
MTBK_RS10140 2178127..2179347 - 1221 WP_003409919.1 TetR family transcriptional regulator Mce3R -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(27-113)

Antitoxin

(44-91)


Sequences


Toxin        


Download         Length: 126 a.a.        Molecular weight: 14397.77 Da        Isoelectric Point: 10.8862

>T47116 WP_010886136.1 NZ_CP007803:2174264-2174641 [Mycobacterium tuberculosis K]
VPPPDPAAMGTWKFFRASVDGRPVFKKEFDKLPDQARAALIVLMQRYLVGDLAAGSIKPIRGDILELRWHEANNHFRVLF
FRWGQHPVALTAFYKNQQKTPKTKIETALDRQKIWKRAFGDTPPI

Download         Length: 378 bp

>T47116 NZ_CP007803:2174264-2174641 [Mycobacterium tuberculosis K]
GTGCCGCCCCCTGATCCAGCCGCCATGGGTACCTGGAAGTTCTTCCGGGCATCTGTGGATGGCCGGCCGGTATTCAAGAA
GGAGTTCGACAAGCTTCCTGATCAGGCCCGGGCCGCGCTGATCGTGCTAATGCAGCGGTATCTCGTCGGCGACCTCGCCG
CAGGGAGCATCAAACCGATTCGTGGCGACATTCTGGAGTTGCGATGGCATGAGGCGAACAACCACTTCCGGGTACTGTTC
TTCCGCTGGGGCCAGCATCCCGTAGCGCTGACAGCGTTCTACAAGAACCAGCAGAAGACTCCCAAGACGAAGATCGAGAC
GGCCCTGGACCGGCAGAAAATCTGGAAAAGAGCCTTCGGCGACACCCCACCGATCTGA

Antitoxin


Download         Length: 150 a.a.        Molecular weight: 16760.16 Da        Isoelectric Point: 8.0771

>AT47116 WP_003409886.1 NZ_CP007803:2174683-2175132 [Mycobacterium tuberculosis K]
MSIDFPLGDDLAGYIAEAIAADPSFKGTLEDAEEARRLVDALIALRKHCQLSQVEVAKRMGVRQPTVSGFEKEPSDPKLS
TLQRYARALDARLRLVLEVPTLREVPTWHRLSSYRGSARDHQVRVGADKEILMQTNWARHISVRQVEVA

Download         Length: 450 bp

>AT47116 NZ_CP007803:2174683-2175132 [Mycobacterium tuberculosis K]
ATGAGCATTGACTTCCCTTTGGGTGACGACCTCGCCGGCTATATTGCCGAGGCGATTGCGGCTGATCCCAGCTTCAAAGG
CACTCTCGAAGACGCCGAGGAGGCACGCAGGCTGGTCGATGCGCTGATTGCGCTGCGCAAGCACTGCCAGCTGAGCCAGG
TTGAGGTTGCTAAGCGTATGGGGGTGCGCCAGCCCACCGTGAGCGGTTTCGAGAAGGAACCCAGCGACCCCAAACTGTCT
ACGCTGCAACGTTATGCCCGTGCATTGGACGCCCGGCTGCGGCTGGTGCTCGAAGTTCCCACGCTTCGCGAAGTGCCTAC
GTGGCATCGGCTCTCCTCTTATCGGGGCTCCGCACGGGACCATCAGGTCCGGGTGGGTGCAGACAAGGAAATCCTGATGC
AGACGAACTGGGCCCGCCACATTTCGGTTCGGCAGGTTGAGGTGGCATGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 7AWK
PDB 7NBU


Antitoxin

Source ID Structure
AlphaFold DB G0TLM0

References