Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2350247..2350749 Replicon chromosome
Accession NZ_CP007799
Organism Escherichia coli Nissle 1917

Toxin (Protein)


Gene name yoeB Uniprot ID S1P977
Locus tag ECOLIN_RS11930 Protein ID WP_000767822.1
Coordinates 2350247..2350501 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag ECOLIN_RS11935 Protein ID WP_001259255.1
Coordinates 2350498..2350749 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECOLIN_RS11915 (ECOLIN_11495) 2346740..2348098 - 1359 WP_000019194.1 putrescine/proton symporter PlaP -
ECOLIN_RS31515 2348088..2348150 - 63 WP_010723108.1 membrane protein YoeI -
ECOLIN_RS11920 (ECOLIN_11500) 2348365..2349294 - 930 WP_000803366.1 LysR family transcriptional regulator -
ECOLIN_RS11925 (ECOLIN_11505) 2349340..2350164 - 825 WP_000754733.1 SDR family oxidoreductase -
ECOLIN_RS11930 (ECOLIN_11510) 2350247..2350501 - 255 WP_000767822.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
ECOLIN_RS11935 (ECOLIN_11515) 2350498..2350749 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
ECOLIN_RS28735 2351032..2351082 + 51 WP_001364200.1 his operon leader peptide -
ECOLIN_RS11940 (ECOLIN_11520) 2351228..2352127 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
ECOLIN_RS11945 (ECOLIN_11525) 2352133..2353437 + 1305 WP_001297912.1 histidinol dehydrogenase -
ECOLIN_RS11950 (ECOLIN_11530) 2353434..2354504 + 1071 WP_000108995.1 histidinol-phosphate transaminase -
ECOLIN_RS11955 (ECOLIN_11535) 2354504..2355571 + 1068 WP_000080122.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T47042 WP_000767822.1 NZ_CP007799:c2350501-2350247 [Escherichia coli Nissle 1917]
VKLIWSEESWDDYLYWQETDKRIVKKINEIIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T47042 NZ_CP007799:c2350501-2350247 [Escherichia coli Nissle 1917]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAAATTATCAAAGATACACGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT47042 WP_001259255.1 NZ_CP007799:c2350749-2350498 [Escherichia coli Nissle 1917]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT47042 NZ_CP007799:c2350749-2350498 [Escherichia coli Nissle 1917]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1P977

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References