Detailed information of TA system TA047010

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 1596305..1596911
• Replicon: chromosome
• Accession: NZ_CP007784
• Organism: Burkholderia cenocepacia strain DDS 22E-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: DM39_RS28110
• Protein ID: WP_040130589.1
• Coordinates: 1596305..1596637 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A132F2X6
• Locus tag: DM39_RS28115
• Protein ID: WP_009691377.1
• Coordinates: 1596624..1596911 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DM39_RS28085	1592958..1593377	-	420	WP_040131926.1	secondary thiamine-phosphate synthase enzyme YjbQ	-
DM39_RS28090	1593388..1593714	-	327	WP_009691383.1	thioredoxin family protein	-
DM39_RS28095	1593912..1594244	+	333	WP_009691382.1	DUF2322 family protein	-
DM39_RS28100	1594409..1595257	+	849	WP_040131927.1	hypothetical protein	-
DM39_RS28105	1595453..1596085	+	633	WP_009691379.1	LysE family translocator	-
DM39_RS28110	1596305..1596637	+	333	WP_040130589.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DM39_RS28115	1596624..1596911	+	288	WP_009691377.1	helix-turn-helix transcriptional regulator	Antitoxin
DM39_RS28120	1596945..1597514	-	570	WP_009691376.1	hypothetical protein	-
DM39_RS28125	1597720..1598178	-	459	WP_009691375.1	Lrp/AsnC family transcriptional regulator	-
DM39_RS28130	1598303..1599043	+	741	WP_009691374.1	AzlC family ABC transporter permease	-
DM39_RS28135	1599036..1599335	+	300	WP_040130590.1	AzlD family protein	-
DM39_RS28140	1599534..1600313	+	780	WP_040130591.1	5'/3'-nucleotidase SurE	-
DM39_RS28145	1600452..1601201	-	750	WP_052102374.1	SDR family oxidoreductase	-
DM39_RS28150	1601198..1601668	-	471	WP_040131930.1	cupin domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (18-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-90)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 12663.90 Da
• Isoelectric Point: 10.1776

>T47010 WP_040130589.1 NZ_CP007784:1596305-1596637 [Burkholderia cenocepacia]
MRFEIRFFSERVEAGIFALPKTLIARFFALADRMERYGPNLGEPHTRAMGDGLYEMRLKGAEGIARVFYCAVVGEQIVML
HCFVKKTPKTPLKELDTARRRLKEVRDGNL

Nucleotide

• Length: 333 bp

>T47010 NZ_CP007784:1596305-1596637 [Burkholderia cenocepacia]
CTGCGCTTTGAAATCCGGTTTTTCAGCGAGCGTGTCGAGGCAGGCATTTTTGCCTTGCCGAAAACGTTGATCGCGCGGTT
CTTCGCACTCGCCGACCGAATGGAACGGTACGGCCCGAATCTGGGCGAGCCTCACACCCGGGCGATGGGCGACGGGTTGT
ATGAAATGCGCCTGAAGGGGGCGGAAGGCATCGCCCGTGTGTTTTACTGCGCAGTCGTCGGCGAGCAGATCGTCATGCTT
CACTGCTTCGTGAAAAAGACGCCGAAAACGCCGTTGAAAGAGCTGGATACCGCGCGCCGCCGATTGAAGGAGGTTCGAGA
TGGCAACCTATAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10473.10 Da
• Isoelectric Point: 10.2231

>AT47010 WP_009691377.1 NZ_CP007784:1596624-1596911 [Burkholderia cenocepacia]
MATYKELRERALADPKVHAEYQRLNREEFALLDAMLAARRAAGLSQADVAERMGTKAPAVTRLERSLATGQHSPSIETLR
KYAAACGKKLVITFA

Nucleotide

• Length: 288 bp

>AT47010 NZ_CP007784:1596624-1596911 [Burkholderia cenocepacia]
ATGGCAACCTATAAGGAACTGCGCGAGCGCGCGCTGGCCGATCCGAAGGTACACGCCGAGTATCAGCGGTTGAATCGCGA
GGAATTCGCGCTGCTCGACGCCATGCTTGCCGCGCGGCGAGCCGCCGGTCTGTCGCAAGCCGATGTTGCGGAGCGCATGG
GCACGAAGGCGCCGGCCGTCACGCGACTCGAACGCTCGCTTGCGACAGGCCAGCACTCGCCGTCGATCGAAACGCTGCGC
AAGTATGCGGCTGCCTGCGGCAAGAAACTCGTGATCACTTTCGCCTGA

Structures

Antitoxin

• AlphaFold DB: A0A132F2X6