Detailed information of TA system TA046973

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 504989..505491
• Replicon: chromosome
• Accession: NZ_CP007744
• Organism: Pectobacterium atrosepticum strain JG10-08

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: Q6DA20
• Locus tag: EV46_RS02255
• Protein ID: WP_011092065.1
• Coordinates: 504989..505243 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: Q6DA19
• Locus tag: EV46_RS02260
• Protein ID: WP_011092066.1
• Coordinates: 505240..505491 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EV46_RS02235	500163..501425	-	1263	WP_039289755.1	L-rhamnose isomerase	-
EV46_RS02240	501422..502912	-	1491	WP_039289758.1	rhamnulokinase	-
EV46_RS02245	503229..504068	+	840	WP_011092063.1	HTH-type transcriptional activator RhaS	-
EV46_RS02250	504093..504947	+	855	WP_039289761.1	helix-turn-helix domain-containing protein	-
EV46_RS02255	504989..505243	-	255	WP_011092065.1	Txe/YoeB family addiction module toxin	Toxin
EV46_RS02260	505240..505491	-	252	WP_011092066.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
EV46_RS02265	505566..507275	-	1710	WP_011092067.1	ABC transporter ATP-binding protein	-
EV46_RS02270	507272..508138	-	867	WP_011092068.1	ABC transporter permease	-
EV46_RS02275	508131..509090	-	960	WP_011092069.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10141.67 Da
• Isoelectric Point: 8.8560

>T46973 WP_011092065.1 NZ_CP007744:c505243-504989 [Pectobacterium atrosepticum]
VKLIWSEEAWEDYLYWQDIDKRTVKKINELIKETRRTPFEGKGKPEPLKHNLAGFWSRRITEAHRLVYAITDDAMMIAAC
RYHY

Nucleotide

• Length: 255 bp

>T46973 NZ_CP007744:c505243-504989 [Pectobacterium atrosepticum]
GTGAAGCTAATCTGGTCAGAAGAAGCATGGGAAGACTACCTGTACTGGCAGGACATCGATAAGCGAACGGTCAAAAAGAT
CAATGAATTAATAAAAGAGACGCGCAGAACACCTTTTGAAGGAAAAGGAAAACCAGAGCCACTTAAACATAACCTTGCTG
GTTTCTGGTCGCGCCGTATCACCGAAGCACATCGTCTGGTGTATGCCATCACAGATGATGCCATGATGATCGCCGCATGC
CGCTACCACTATTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9397.66 Da
• Isoelectric Point: 4.6119

>AT46973 WP_011092066.1 NZ_CP007744:c505491-505240 [Pectobacterium atrosepticum]
MRTISYSEARQNLSATMMKTVEDRAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANAKRLMNSIESLKAGNSEERD
IIE

Nucleotide

• Length: 252 bp

>AT46973 NZ_CP007744:c505491-505240 [Pectobacterium atrosepticum]
ATGCGTACAATTAGCTACAGCGAAGCCCGTCAAAATCTATCAGCGACAATGATGAAAACCGTCGAAGATCGTGCTCCCAT
TCTGATCACTCGACAGAATGGTGAGGCCTGCGTGCTTATGTCTCTGGAAGAGTATAATTCTCTAGAGGAAACAGCATATT
TACTGCGTTCACCAGCAAATGCAAAAAGGCTGATGAATTCAATTGAAAGCCTTAAGGCTGGCAATAGCGAAGAAAGAGAT
ATCATTGAGTGA

Structures

Toxin

• AlphaFold DB: Q6DA20

Antitoxin

• AlphaFold DB: Q6DA19