Detailed information of TA system TA046972

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 423967..424691
• Replicon: chromosome
• Accession: NZ_CP007744
• Organism: Pectobacterium atrosepticum strain JG10-08

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q6DA89
• Locus tag: EV46_RS01930
• Protein ID: WP_011091996.1
• Coordinates: 423967..424278 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EV46_RS01935
• Protein ID: WP_011091997.1
• Coordinates: 424275..424691 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EV46_RS01915	420123..421658	-	1536	WP_011091993.1	carbohydrate porin	-
EV46_RS01920	421849..422784	-	936	WP_011091994.1	aminoimidazole riboside kinase	-
EV46_RS01925	423256..423807	+	552	WP_039294652.1	polymer-forming cytoskeletal protein	-
EV46_RS01930	423967..424278	+	312	WP_011091996.1	type II toxin-antitoxin system HigB family toxin	Toxin
EV46_RS01935	424275..424691	+	417	WP_011091997.1	helix-turn-helix domain-containing protein	Antitoxin
EV46_RS01940	424894..425352	+	459	WP_011091998.1	hypothetical protein	-
EV46_RS01945	425443..426795	-	1353	WP_011091999.1	aspartate aminotransferase family protein	-
EV46_RS01950	426829..428109	-	1281	WP_011092000.1	MFS transporter	-
EV46_RS01955	428263..428709	+	447	WP_011092001.1	helix-turn-helix transcriptional regulator	-
EV46_RS01960	428719..429213	+	495	WP_011092002.1	YhbP family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (83-136)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12431.33 Da
• Isoelectric Point: 10.0380

>T46972 WP_011091996.1 NZ_CP007744:423967-424278 [Pectobacterium atrosepticum]
MHVISRAPFDAATNQFPNQAVALADLYRVIKREMYATPEDMKKRFPSLDRMKYREKWWVIDIGGGHLRVMFFADFERGKI
FIKHITSHAEYDRLTEYYRRNKE

Nucleotide

• Length: 312 bp

>T46972 NZ_CP007744:423967-424278 [Pectobacterium atrosepticum]
ATGCATGTGATTTCGCGCGCACCTTTTGATGCTGCAACCAACCAGTTTCCGAATCAGGCGGTAGCACTCGCTGACCTATA
TCGGGTTATAAAACGCGAAATGTATGCAACGCCGGAGGATATGAAAAAACGCTTTCCCAGCTTAGATAGGATGAAATATC
GAGAAAAGTGGTGGGTTATTGATATCGGTGGTGGGCATCTTCGAGTGATGTTTTTTGCTGATTTTGAGCGAGGGAAAATC
TTCATCAAGCACATAACATCCCATGCAGAGTACGACAGGCTGACAGAGTATTACCGGAGGAATAAAGAATGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15395.73 Da
• Isoelectric Point: 4.7943

>AT46972 WP_011091997.1 NZ_CP007744:424275-424691 [Pectobacterium atrosepticum]
MMYADAIKAANDLTSIVPFLGGSTSRKDYEDALKLTEYLIESEPDSSLIDMLTAKIDKYENEAPEFAAFNERIKALPAGI
ALLRVLMDQHQLMQSDFEAEIGKKSLVSRILNGERTLTLAHMRALAKRFNIPVSAFVD

Nucleotide

• Length: 417 bp

>AT46972 NZ_CP007744:424275-424691 [Pectobacterium atrosepticum]
ATGATGTATGCAGATGCCATCAAGGCAGCTAACGATCTGACGAGTATCGTACCGTTCCTCGGGGGCAGCACCTCTCGTAA
GGATTATGAGGATGCACTGAAACTCACTGAATATCTGATCGAGAGTGAGCCTGATAGTTCGCTAATCGATATGCTGACTG
CCAAAATTGATAAATACGAAAATGAGGCGCCGGAGTTTGCAGCCTTCAACGAACGTATTAAAGCACTCCCTGCCGGTATC
GCTTTGCTACGTGTCCTCATGGATCAGCACCAGCTAATGCAGAGTGATTTTGAAGCTGAGATCGGTAAAAAGTCTCTGGT
ATCCCGTATTCTAAACGGTGAGCGTACGTTAACACTTGCTCATATGAGAGCTCTCGCTAAGCGATTCAATATTCCCGTGA
GTGCTTTTGTTGATTAA

Structures

Toxin

• AlphaFold DB: Q6DA89