Detailed information of TA system TA046967

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 11781..12505
• Replicon: plasmid pKPN-b0b
• Accession: NZ_CP007736
• Organism: Klebsiella pneumoniae subsp. pneumoniae KPNIH27

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A2J4YLV3
• Locus tag: KPNIH27_RS30435
• Protein ID: WP_023292165.1
• Coordinates: 12194..12505 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KPNIH27_RS30430
• Protein ID: WP_020801939.1
• Coordinates: 11781..12197 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KPNIH27_RS30405	7548..8315	-	768	WP_042922548.1	DUF945 domain-containing protein	-
KPNIH27_RS30410	9142..9975	-	834	WP_042922549.1	type I restriction-modification system subunit M	-
KPNIH27_RS30415	10024..10170	-	147	WP_004152750.1	hypothetical protein	-
KPNIH27_RS30420	10265..10612	-	348	WP_020804846.1	hypothetical protein	-
KPNIH27_RS30425	10679..11059	-	381	WP_020802391.1	hypothetical protein	-
KPNIH27_RS30430	11781..12197	-	417	WP_020801939.1	helix-turn-helix domain-containing protein	Antitoxin
KPNIH27_RS30435	12194..12505	-	312	WP_023292165.1	type II toxin-antitoxin system HigB family toxin	Toxin
KPNIH27_RS30440	12612..12836	-	225	WP_042922612.1	hypothetical protein	-
KPNIH27_RS30445	12847..13053	-	207	WP_042922554.1	hypothetical protein	-
KPNIH27_RS30450	13690..14040	-	351	WP_004152758.1	hypothetical protein	-
KPNIH27_RS30455	14037..14309	-	273	WP_042922555.1	hypothetical protein	-
KPNIH27_RS30460	14667..15389	-	723	WP_020804573.1	plasmid SOS inhibition protein A	-
KPNIH27_RS30465	15386..15817	-	432	WP_020804574.1	conjugation system SOS inhibitor PsiB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..113440	113440

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (84-133)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12497.32 Da
• Isoelectric Point: 9.9242

>T46967 WP_023292165.1 NZ_CP007736:c12505-12194 [Klebsiella pneumoniae subsp. pneumoniae KPNIH27]
VHVISRAPFDEAARHYPNDAAAIDDTYRVLKRVVAKKPDELKRYFTSLDRMKYREKWWVIDIGGNNLRMMFFADFERGKI
FVKHITTHAEYDRLTKYYREHKE

Nucleotide

• Length: 312 bp

>T46967 NZ_CP007736:c12505-12194 [Klebsiella pneumoniae subsp. pneumoniae KPNIH27]
GTGCATGTTATATCACGAGCCCCTTTTGATGAGGCGGCGAGACACTATCCGAATGATGCTGCGGCCATCGATGACACCTA
TCGTGTACTAAAGCGGGTTGTCGCAAAAAAACCGGATGAGCTAAAGCGATACTTTACTAGTCTGGATCGCATGAAATACC
GGGAAAAATGGTGGGTTATCGATATCGGTGGTAATAACCTTAGAATGATGTTTTTTGCTGATTTTGAGCGCGGAAAGATA
TTCGTCAAACACATAACCACCCATGCTGAATATGATCGCTTAACGAAATATTATCGGGAGCACAAAGAATGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15380.49 Da
• Isoelectric Point: 4.5542

>AT46967 WP_020801939.1 NZ_CP007736:c12197-11781 [Klebsiella pneumoniae subsp. pneumoniae KPNIH27]
MIFSDAIKAANDLASIVPLLGGSSSRKDYEEALKLVEYLLEHDPDSPLVDMLTARIDAWEDNAVEFEEFNTRIEAGKNGV
SLLRVLMQQHGLSQSDFENEIGKKSLVSRILSGERSLTLDHMRALANRFQIPVSMFVG

Nucleotide

• Length: 417 bp

>AT46967 NZ_CP007736:c12197-11781 [Klebsiella pneumoniae subsp. pneumoniae KPNIH27]
ATGATTTTTAGCGATGCCATTAAAGCGGCGAATGACCTGGCGAGTATTGTCCCTCTGCTGGGTGGCAGCTCGTCCCGCAA
AGATTACGAAGAGGCGCTTAAACTCGTTGAATATCTTCTGGAACATGACCCGGACAGCCCGCTGGTTGATATGCTGACTG
CCCGCATCGATGCCTGGGAAGATAACGCGGTGGAGTTTGAAGAATTTAATACGCGCATCGAAGCAGGCAAAAACGGTGTA
TCGTTGCTGCGGGTTCTTATGCAGCAACACGGGCTTTCACAGTCCGATTTTGAAAACGAGATAGGCAAAAAATCGCTCGT
CAGCCGGATCCTCAGTGGTGAACGCAGTCTGACCCTTGATCACATGCGGGCGCTGGCAAATCGATTTCAAATCCCGGTCT
CGATGTTTGTTGGCTGA

Structures

Toxin

• AlphaFold DB: A0A2J4YLV3