Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 4866628..4867214 Replicon chromosome
Accession NZ_CP007731
Organism Klebsiella pneumoniae subsp. pneumoniae KPNIH27

Toxin (Protein)


Gene name doc Uniprot ID W8VD46
Locus tag KPNIH27_RS24335 Protein ID WP_002920800.1
Coordinates 4866628..4866996 (-) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID W9B1V1
Locus tag KPNIH27_RS24340 Protein ID WP_004174006.1
Coordinates 4866993..4867214 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KPNIH27_RS24315 4862131..4863201 - 1071 WP_004150074.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -
KPNIH27_RS24320 4863203..4864048 - 846 WP_004145129.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
KPNIH27_RS24325 4864045..4864932 - 888 WP_002920792.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
KPNIH27_RS24330 4865039..4866355 - 1317 WP_032442539.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
KPNIH27_RS24335 4866628..4866996 - 369 WP_002920800.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
KPNIH27_RS24340 4866993..4867214 - 222 WP_004174006.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
KPNIH27_RS24345 4867378..4868091 - 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
KPNIH27_RS24350 4868093..4868860 - 768 WP_002920803.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
KPNIH27_RS24355 4868857..4870134 - 1278 WP_004174005.1 branched chain amino acid ABC transporter permease LivM -
KPNIH27_RS24360 4870131..4871057 - 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 4861394..4883432 22038


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(13-86)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13552.92 Da        Isoelectric Point: 8.6410

>T46960 WP_002920800.1 NZ_CP007731:c4866996-4866628 [Klebsiella pneumoniae subsp. pneumoniae KPNIH27]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T46960 NZ_CP007731:c4866996-4866628 [Klebsiella pneumoniae subsp. pneumoniae KPNIH27]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8151.15 Da        Isoelectric Point: 4.7769

>AT46960 WP_004174006.1 NZ_CP007731:c4867214-4866993 [Klebsiella pneumoniae subsp. pneumoniae KPNIH27]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT46960 NZ_CP007731:c4867214-4866993 [Klebsiella pneumoniae subsp. pneumoniae KPNIH27]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GUD1

Antitoxin

Source ID Structure
AlphaFold DB A0A5E5YJY7

References