Detailed information of TA system TA046959

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 4840379..4841025
• Replicon: chromosome
• Accession: NZ_CP007731
• Organism: Klebsiella pneumoniae subsp. pneumoniae KPNIH27

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: KPNIH27_RS24225
• Protein ID: WP_023317328.1
• Coordinates: 4840678..4841025 (-)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KPNIH27_RS24220
• Protein ID: WP_032412314.1
• Coordinates: 4840379..4840678 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KPNIH27_RS24200	4836585..4837343	-	759	WP_002920548.1	DeoR/GlpR family transcriptional regulator	-
KPNIH27_RS24205	4837393..4838223	-	831	WP_004151408.1	rhomboid family intramembrane serine protease GlpG	-
KPNIH27_RS24210	4838274..4838603	-	330	WP_002920552.1	thiosulfate sulfurtransferase GlpE	-
KPNIH27_RS24215	4838808..4840316	+	1509	WP_032412313.1	glycerol-3-phosphate dehydrogenase	-
KPNIH27_RS24220	4840379..4840678	-	300	WP_032412314.1	XRE family transcriptional regulator	Antitoxin
KPNIH27_RS24225	4840678..4841025	-	348	WP_023317328.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KPNIH27_RS24230	4841201..4843648	-	2448	WP_002920561.1	glycogen phosphorylase	-
KPNIH27_RS24235	4843666..4845099	-	1434	WP_002920564.1	glycogen synthase GlgA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-107)

Antitoxin

• HigA2-like, helix-turn-helix domain (9-87)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13562.60 Da
• Isoelectric Point: 6.2327

>T46959 WP_023317328.1 NZ_CP007731:c4841025-4840678 [Klebsiella pneumoniae subsp. pneumoniae KPNIH27]
MWDVETTDTFDTWFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRAFFAFDPLRKAI
ILCAGNKDGMNEKRFYKEMITLADREFSQHLTKER

Nucleotide

• Length: 348 bp

>T46959 NZ_CP007731:c4841025-4840678 [Klebsiella pneumoniae subsp. pneumoniae KPNIH27]
ATGTGGGATGTAGAAACGACGGATACGTTTGATACCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTCCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
ATTTTATGTGCGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10828.64 Da
• Isoelectric Point: 10.1464

>AT46959 WP_032412314.1 NZ_CP007731:c4840678-4840379 [Klebsiella pneumoniae subsp. pneumoniae KPNIH27]
MATLKELMAKQSPESQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGIKQPTIAKMEQADNDPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Nucleotide

• Length: 300 bp

>AT46959 NZ_CP007731:c4840678-4840379 [Klebsiella pneumoniae subsp. pneumoniae KPNIH27]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGAAAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGATAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA