Detailed information of TA system TA046947

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 1350217..1350814
• Replicon: chromosome
• Accession: NZ_CP007731
• Organism: Klebsiella pneumoniae subsp. pneumoniae KPNIH27

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A9J6S5A1
• Locus tag: KPNIH27_RS06635
• Protein ID: WP_004893639.1
• Coordinates: 1350217..1350534 (+)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: R4YH91
• Locus tag: KPNIH27_RS06640
• Protein ID: WP_004142561.1
• Coordinates: 1350527..1350814 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KPNIH27_RS06620	1345921..1347348	-	1428	WP_009308097.1	MFS transporter	-
KPNIH27_RS06625	1347457..1348323	+	867	WP_004151823.1	helix-turn-helix transcriptional regulator	-
KPNIH27_RS06630	1348989..1350032	+	1044	WP_032410657.1	DUF2157 domain-containing protein	-
KPNIH27_RS06635	1350217..1350534	+	318	WP_004893639.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KPNIH27_RS06640	1350527..1350814	+	288	WP_004142561.1	helix-turn-helix transcriptional regulator	Antitoxin
KPNIH27_RS06645	1351079..1351732	-	654	WP_004178896.1	oxygen-insensitive NAD(P)H nitroreductase	-
KPNIH27_RS06650	1351852..1352220	-	369	WP_032421096.1	MmcQ/YjbR family DNA-binding protein	-
KPNIH27_RS06655	1352220..1352801	-	582	WP_032410661.1	TetR/AcrR family transcriptional regulator	-
KPNIH27_RS06660	1352981..1354096	+	1116	WP_020316605.1	MBL fold metallo-hydrolase	-
KPNIH27_RS06665	1354127..1354468	+	342	WP_002893035.1	RamA family antibiotic efflux transcriptional regulator	-
KPNIH27_RS06670	1354486..1354734	-	249	WP_032410665.1	DUF1158 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-88)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12100.35 Da
• Isoelectric Point: 11.2767

>T46947 WP_004893639.1 NZ_CP007731:1350217-1350534 [Klebsiella pneumoniae subsp. pneumoniae KPNIH27]
MFRLVVHVDVKKELQALPSIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Nucleotide

• Length: 318 bp

>T46947 NZ_CP007731:1350217-1350534 [Klebsiella pneumoniae subsp. pneumoniae KPNIH27]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGTCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10771.46 Da
• Isoelectric Point: 10.2212

>AT46947 WP_004142561.1 NZ_CP007731:1350527-1350814 [Klebsiella pneumoniae subsp. pneumoniae KPNIH27]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Nucleotide

• Length: 288 bp

>AT46947 NZ_CP007731:1350527-1350814 [Klebsiella pneumoniae subsp. pneumoniae KPNIH27]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Structures

Antitoxin

• AlphaFold DB: A0A0S3DIQ1