Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/Txe-RelB
Location 2545704..2546227 Replicon chromosome
Accession NZ_CP007657
Organism Staphylococcus aureus strain V2200

Toxin (Protein)


Gene name yoeB Uniprot ID X5DYX7
Locus tag EP20_RS13070 Protein ID WP_000074048.1
Coordinates 2545704..2545970 (-) Length 89 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID W8TQT5
Locus tag EP20_RS13075 Protein ID WP_000587616.1
Coordinates 2545970..2546227 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EP20_RS13050 2541011..2542198 - 1188 WP_000026194.1 MFS transporter -
EP20_RS13055 2542594..2543370 - 777 WP_000072147.1 iron export ABC transporter permease subunit FetB -
EP20_RS13060 2543363..2544025 - 663 WP_000923520.1 ATP-binding cassette domain-containing protein -
EP20_RS13065 2544273..2545349 - 1077 WP_001076671.1 M42 family metallopeptidase -
EP20_RS13070 2545704..2545970 - 267 WP_000074048.1 Txe/YoeB family addiction module toxin Toxin
EP20_RS13075 2545970..2546227 - 258 WP_000587616.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
EP20_RS13085 2546586..2547041 + 456 WP_000732082.1 DUF1307 domain-containing protein -
EP20_RS13090 2547209..2548787 - 1579 Protein_2468 FMN-binding glutamate synthase family protein -
EP20_RS13095 2548982..2549731 + 750 WP_000072443.1 hypothetical protein -
EP20_RS13100 2549955..2551148 - 1194 WP_000675401.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(25-87)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-68)


Sequences

Toxin        


Download         Length: 89 a.a.        Molecular weight: 10648.23 Da        Isoelectric Point: 10.1384

>T46794 WP_000074048.1 NZ_CP007657:c2545970-2545704 [Staphylococcus aureus]
MSNYTVKIKNSAKSDLKKIKHSYLKKSFLEIVETLKNDPYKITQSFEKLEPKYLERYSRRINHQHRVVYTVDDRNKEVLI
LSAWSHYD

Download         Length: 267 bp

>T46794 NZ_CP007657:c2545970-2545704 [Staphylococcus aureus]
ATGAGCAATTACACGGTTAAGATTAAAAATTCAGCGAAATCAGATTTAAAGAAAATAAAACATTCTTATTTAAAGAAGTC
ATTTTTAGAAATTGTTGAGACTTTAAAAAATGATCCGTATAAAATAACACAATCTTTTGAAAAATTAGAGCCTAAATATT
TAGAGCGATATTCAAGAAGAATTAACCATCAGCACAGGGTCGTCTATACCGTAGATGATCGAAATAAAGAAGTATTAATA
CTATCGGCATGGTCACATTATGATTAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9774.73 Da        Isoelectric Point: 4.1353

>AT46794 WP_000587616.1 NZ_CP007657:c2546227-2545970 [Staphylococcus aureus]
MIITSPTEARKDFYQLLKNVNNNHEPIYISGNNAENNAVIIGLEDWKSIQETIYLESTGTMDKVREREKDNSGTTNIDDI
DWDNL

Download         Length: 258 bp

>AT46794 NZ_CP007657:c2546227-2545970 [Staphylococcus aureus]
ATGATTATCACTAGCCCTACAGAAGCGAGAAAAGATTTTTATCAATTACTAAAAAATGTTAATAATAATCACGAACCAAT
TTATATTAGTGGCAATAATGCCGAAAATAATGCTGTGATTATAGGTTTAGAAGATTGGAAAAGTATACAAGAGACAATAT
ATCTTGAATCTACTGGTACAATGGACAAAGTAAGAGAAAGAGAAAAAGATAATAGTGGTACAACAAATATAGATGATATT
GATTGGGATAATCTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7V6W
PDB 7V5Y
PDB 7V5Z

Antitoxin

Source ID Structure
PDB 7V6W
PDB 7V5Z
PDB 7V5Y
AlphaFold DB W8TQT5

References