Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | higBA (relBE)/COG4683-HTH_37 |
| Location | 31582..32239 | Replicon | plasmid unnamed |
| Accession | NZ_CP007636 | ||
| Organism | Vibrio cholerae strain 2012EL-2176 | ||
Toxin (Protein)
| Gene name | higB | Uniprot ID | U3PDC3 |
| Locus tag | EN18_RS19300 | Protein ID | WP_000270043.1 |
| Coordinates | 31889..32239 (-) | Length | 117 a.a. |
Antitoxin (Protein)
| Gene name | higA | Uniprot ID | - |
| Locus tag | EN18_RS19295 | Protein ID | WP_000124640.1 |
| Coordinates | 31582..31884 (-) | Length | 101 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| EN18_RS19245 | 27207..27635 | + | 429 | WP_000591074.1 | hypothetical protein | - |
| EN18_RS19250 | 27692..28051 | + | 360 | WP_000422768.1 | hypothetical protein | - |
| EN18_RS19255 | 28051..28497 | + | 447 | WP_000919345.1 | Fe3+-siderophore ABC transporter permease | - |
| EN18_RS19260 | 28494..29012 | + | 519 | WP_000210756.1 | nitrite reductase | - |
| EN18_RS19265 | 29012..29242 | + | 231 | WP_000972663.1 | hypothetical protein | - |
| EN18_RS19270 | 29229..30086 | + | 858 | WP_001167032.1 | hypothetical protein | - |
| EN18_RS19280 | 30317..30844 | + | 528 | WP_001236377.1 | thermonuclease family protein | - |
| EN18_RS19285 | 30902..31174 | + | 273 | WP_001043047.1 | HU family DNA-binding protein | - |
| EN18_RS19290 | 31262..31555 | + | 294 | WP_001239997.1 | chromosome segregation protein ParM | - |
| EN18_RS19295 | 31582..31884 | - | 303 | WP_000124640.1 | XRE family transcriptional regulator | Antitoxin |
| EN18_RS19300 | 31889..32239 | - | 351 | WP_000270043.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| EN18_RS19305 | 32402..32950 | + | 549 | WP_001061574.1 | transcriptional regulator | - |
| EN18_RS19315 | 33291..33485 | + | 195 | WP_000343597.1 | hypothetical protein | - |
| EN18_RS19320 | 33496..33867 | + | 372 | WP_000516916.1 | hypothetical protein | - |
| EN18_RS19325 | 33860..34330 | + | 471 | WP_001281821.1 | hypothetical protein | - |
| EN18_RS19330 | 34345..34680 | - | 336 | WP_000683476.1 | hypothetical protein | - |
| EN18_RS19335 | 34777..35265 | + | 489 | WP_001273096.1 | DUF1643 domain-containing protein | - |
| EN18_RS19340 | 35268..35765 | + | 498 | WP_000062185.1 | hypothetical protein | - |
| EN18_RS19345 | 35980..36684 | - | 705 | WP_001067855.1 | IS6-like element IS26 family transposase | - |
| EN18_RS19350 | 36747..37202 | + | 456 | Protein_49 | recombinase family protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | blaTEM-1D / aac(3)-IIa / floR / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / blaCMY-2 / ARR-3 / dfrA27 / aadA16 / qacE / sul1 / blaCTX-M-2 / mph(A) | - | 1..167267 | 167267 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 117 a.a. Molecular weight: 13328.21 Da Isoelectric Point: 5.2421
>T46767 WP_000270043.1 NZ_CP007636:c32239-31889 [Vibrio cholerae]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE
Download Length: 351 bp
>T46767 NZ_CP007636:c32239-31889 [Vibrio cholerae]
ATGTGGGTCATCGAGACAACCGACACCTTTGATGAGTGGTTCGATGCTCTGGATGATACCGATAGAGCAAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGCCCCATGCTGTCGAGGCCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTTCGGGTCCAAAGCAAAGGAGATCCTATCAGAGCGTTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGTGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA
ATGTGGGTCATCGAGACAACCGACACCTTTGATGAGTGGTTCGATGCTCTGGATGATACCGATAGAGCAAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGCCCCATGCTGTCGAGGCCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTTCGGGTCCAAAGCAAAGGAGATCCTATCAGAGCGTTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGTGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA
Antitoxin
Download Length: 101 a.a. Molecular weight: 11006.73 Da Isoelectric Point: 7.2036
>AT46767 WP_000124640.1 NZ_CP007636:c31884-31582 [Vibrio cholerae]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV
Download Length: 303 bp
>AT46767 NZ_CP007636:c31884-31582 [Vibrio cholerae]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|