Detailed information of TA system TA046730

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ(toxin)
• Location: 359309..359897
• Replicon: chromosome
• Accession: NZ_CP007605
• Organism: Helicobacter pylori strain BM012B

Toxin (Protein)

• Gene name: TfiT
• Uniprot ID: M3P300
• Locus tag: EG66_RS01910
• Protein ID: WP_000921941.1
• Coordinates: 359309..359590 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: TfiA
• Uniprot ID: V5NLP9
• Locus tag: EG66_RS01915
• Protein ID: WP_023591735.1
• Coordinates: 359583..359897 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EG66_RS01880	354429..355082	-	654	WP_023591539.1	IS607-like element IS607 family transposase	-
EG66_RS01885	355163..355876	-	714	Protein_349	competence protein	-
EG66_RS01890	355919..356389	-	471	Protein_350	hypothetical protein	-
EG66_RS01895	356482..357189	+	708	WP_038419699.1	hypothetical protein	-
EG66_RS01900	357194..357565	+	372	WP_038419701.1	hypothetical protein	-
EG66_RS08295	357662..359044	+	1383	Protein_353	type IV secretory system conjugative DNA transfer family protein	-
EG66_RS01910	359309..359590	-	282	WP_000921941.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	Toxin
EG66_RS01915	359583..359897	-	315	WP_023591735.1	hypothetical protein	Antitoxin
EG66_RS01920	360235..362145	+	1911	WP_038420352.1	relaxase/mobilization nuclease domain-containing protein	-
EG66_RS01925	362170..363354	-	1185	WP_023591737.1	hypothetical protein	-
EG66_RS01930	363398..363682	-	285	WP_000394639.1	hypothetical protein	-
EG66_RS01935	363763..364419	-	657	WP_023591738.1	ParA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	350450..379590	29140

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (3-92)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10891.80 Da
• Isoelectric Point: 9.5726

>T46730 WP_000921941.1 NZ_CP007605:c359590-359309 [Helicobacter pylori]
MLKVRTKKDFLKDFNKHILSGRITESDVTSVVDCLKEQKPLQQKYCDHALSGNLKGLRECHVKPNLLLIYEIKKQENELV
LLRLDTHSELFKK

Nucleotide

• Length: 282 bp

>T46730 NZ_CP007605:c359590-359309 [Helicobacter pylori]
ATGCTTAAAGTCAGAACTAAGAAAGATTTTCTTAAAGACTTTAATAAGCATATCTTATCTGGGCGTATTACAGAAAGCGA
TGTTACAAGCGTTGTTGATTGCCTAAAAGAACAAAAACCACTCCAACAAAAATATTGCGACCACGCTTTGAGTGGCAATC
TTAAAGGGTTGAGAGAATGCCATGTCAAGCCAAACTTGTTATTGATTTATGAAATCAAAAAACAAGAAAACGAACTTGTT
TTATTGCGTCTAGACACTCATAGCGAGCTATTTAAAAAGTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12296.75 Da
• Isoelectric Point: 5.6815

>AT46730 WP_023591735.1 NZ_CP007605:c359897-359583 [Helicobacter pylori]
MSNIITDYSQYNEKQLHNFLNSIEKQLLKAEENKNKAIKKIQECELQERMIRQVLAQKHSQEKEPTQSLLNTIASKDDPE
YDVSFGDFNDFLQIAKQERERHNA

Nucleotide

• Length: 315 bp

>AT46730 NZ_CP007605:c359897-359583 [Helicobacter pylori]
ATGTCAAATATTATTACAGATTACTCGCAATACAATGAAAAGCAACTGCACAATTTCTTAAACTCCATTGAAAAACAATT
GCTTAAGGCAGAAGAGAATAAAAATAAAGCGATAAAAAAAATCCAAGAGTGTGAATTGCAAGAACGAATGATCAGACAAG
TTTTAGCCCAAAAACATTCTCAAGAAAAGGAACCCACACAAAGTTTGTTAAATACGATCGCCTCAAAAGATGACCCAGAA
TACGATGTTTCGTTTGGAGACTTCAATGATTTTTTACAAATAGCAAAACAAGAAAGAGAAAGGCATAATGCTTAA

Structures

Toxin

• AlphaFold DB: M3P300

Antitoxin

• AlphaFold DB: V5NLP9