Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2322887..2323389 Replicon chromosome
Accession NZ_CP007594
Organism Escherichia coli strain SEC470

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag DP32_RS11800 Protein ID WP_000767829.1
Coordinates 2322887..2323141 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag DP32_RS11805 Protein ID WP_001259255.1
Coordinates 2323138..2323389 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DP32_RS11775 2317902..2318129 - 228 WP_000985281.1 sulfurtransferase TusA family protein -
DP32_RS11780 2318143..2319201 - 1059 WP_000492300.1 transport protein YeeE -
DP32_RS11785 2319380..2320738 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
DP32_RS29255 2320728..2320790 - 63 WP_010723108.1 membrane protein YoeI -
DP32_RS11790 2321005..2321934 - 930 WP_000803380.1 LysR family transcriptional regulator -
DP32_RS11795 2321980..2322804 - 825 WP_000754737.1 SDR family oxidoreductase -
DP32_RS11800 2322887..2323141 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
DP32_RS11805 2323138..2323389 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
DP32_RS27190 2323672..2323722 + 51 WP_001368802.1 his operon leader peptide -
DP32_RS11810 2323868..2324767 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
DP32_RS11815 2324773..2326077 + 1305 WP_014639951.1 histidinol dehydrogenase -
DP32_RS11820 2326074..2327144 + 1071 WP_000108952.1 histidinol-phosphate transaminase -
DP32_RS11825 2327144..2328211 + 1068 WP_000080076.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T46702 WP_000767829.1 NZ_CP007594:c2323141-2322887 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T46702 NZ_CP007594:c2323141-2322887 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT46702 WP_001259255.1 NZ_CP007594:c2323389-2323138 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT46702 NZ_CP007594:c2323389-2323138 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References