Detailed information of TA system TA046677

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4650277..4650879
• Replicon: chromosome
• Accession: NZ_CP007592
• Organism: Escherichia coli O157:H16 strain Santai

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: CH99_RS22775
• Protein ID: WP_000897305.1
• Coordinates: 4650277..4650588 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CH99_RS22780
• Protein ID: WP_000356397.1
• Coordinates: 4650589..4650879 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CH99_RS22745	4645307..4646092	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
CH99_RS22750	4646191..4646790	+	600	WP_001295269.1	glucose-1-phosphatase	-
CH99_RS22755	4646784..4647656	+	873	WP_000920762.1	virulence factor BrkB family protein	-
CH99_RS22760	4647653..4648090	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
CH99_RS22765	4648135..4649076	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
CH99_RS22770	4649140..4650048	-	909	WP_001162704.1	alpha/beta hydrolase	-
CH99_RS22775	4650277..4650588	+	312	WP_000897305.1	hypothetical protein	Toxin
CH99_RS22780	4650589..4650879	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
CH99_RS22785	4651465..4651683	+	219	WP_001315930.1	ribbon-helix-helix domain-containing protein	-
CH99_RS22790	4651903..4652145	+	243	WP_001087409.1	hypothetical protein	-
CH99_RS22800	4652475..4653404	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
CH99_RS22805	4653401..4654036	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
CH99_RS22810	4654033..4654935	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T46677 WP_000897305.1 NZ_CP007592:4650277-4650588 [Escherichia coli O157:H16]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T46677 NZ_CP007592:4650277-4650588 [Escherichia coli O157:H16]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT46677 WP_000356397.1 NZ_CP007592:4650589-4650879 [Escherichia coli O157:H16]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT46677 NZ_CP007592:4650589-4650879 [Escherichia coli O157:H16]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7