Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2473384..2473886 Replicon chromosome
Accession NZ_CP007592
Organism Escherichia coli O157:H16 strain Santai

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag CH99_RS12295 Protein ID WP_000767829.1
Coordinates 2473384..2473638 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag CH99_RS12300 Protein ID WP_001259255.1
Coordinates 2473635..2473886 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CH99_RS12270 2468399..2468626 - 228 WP_000234896.1 sulfurtransferase TusA -
CH99_RS12275 2468640..2469698 - 1059 WP_000492340.1 transport protein YeeE -
CH99_RS12280 2469877..2471235 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
CH99_RS28055 2471225..2471287 - 63 WP_010723108.1 membrane protein YoeI -
CH99_RS12285 2471502..2472431 - 930 WP_000803366.1 LysR family transcriptional regulator -
CH99_RS12290 2472477..2473301 - 825 WP_000754752.1 SDR family oxidoreductase -
CH99_RS12295 2473384..2473638 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
CH99_RS12300 2473635..2473886 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
CH99_RS26130 2474170..2474220 + 51 WP_001364200.1 his operon leader peptide -
CH99_RS12305 2474366..2475265 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
CH99_RS12310 2475271..2476575 + 1305 WP_039023537.1 histidinol dehydrogenase -
CH99_RS12315 2476572..2477642 + 1071 WP_039023536.1 histidinol-phosphate transaminase -
CH99_RS12320 2477642..2478709 + 1068 WP_000080077.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T46670 WP_000767829.1 NZ_CP007592:c2473638-2473384 [Escherichia coli O157:H16]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T46670 NZ_CP007592:c2473638-2473384 [Escherichia coli O157:H16]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAGCATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT46670 WP_001259255.1 NZ_CP007592:c2473886-2473635 [Escherichia coli O157:H16]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT46670 NZ_CP007592:c2473886-2473635 [Escherichia coli O157:H16]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References