Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 526019..526560 Replicon chromosome
Accession NZ_CP007592
Organism Escherichia coli O157:H16 strain Santai

Toxin (Protein)


Gene name yafQ Uniprot ID Q32JN3
Locus tag CH99_RS02515 Protein ID WP_000615980.1
Coordinates 526019..526297 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q32JN2
Locus tag CH99_RS02520 Protein ID WP_000729709.1
Coordinates 526300..526560 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CH99_RS02500 523601..524179 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
CH99_RS02505 524385..525152 + 768 WP_000333379.1 class II glutamine amidotransferase -
CH99_RS02510 525123..525863 - 741 WP_001225679.1 murein L,D-transpeptidase -
CH99_RS02515 526019..526297 - 279 WP_000615980.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
CH99_RS02520 526300..526560 - 261 WP_000729709.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
CH99_RS02525 526770..527519 + 750 WP_000056850.1 C40 family peptidase -
CH99_RS02530 527695..528192 + 498 WP_021556447.1 REP-associated tyrosine transposase RayT -
CH99_RS02535 528385..530478 - 2094 WP_021556448.1 flagellar biosynthesis protein FlhA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 519051..528192 9141


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(5-92)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10830.62 Da        Isoelectric Point: 10.0702

>T46659 WP_000615980.1 NZ_CP007592:c526297-526019 [Escherichia coli O157:H16]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWLLIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T46659 NZ_CP007592:c526297-526019 [Escherichia coli O157:H16]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGCTCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9333.73 Da        Isoelectric Point: 5.8129

>AT46659 WP_000729709.1 NZ_CP007592:c526560-526300 [Escherichia coli O157:H16]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVGVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT46659 NZ_CP007592:c526560-526300 [Escherichia coli O157:H16]
ATGGCTGCAAATGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGGTGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090NH48

Antitoxin

Source ID Structure
AlphaFold DB A0A829FY17

References