Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | higBA (relBE)/COG4683-HTH_37 |
| Location | 13609..14266 | Replicon | plasmid pKEC-a3c |
| Accession | NZ_CP007558 | ||
| Organism | Citrobacter freundii CFNIH1 | ||
Toxin (Protein)
| Gene name | higB | Uniprot ID | U3PDC3 |
| Locus tag | CFNIH1_RS24460 | Protein ID | WP_000270043.1 |
| Coordinates | 13916..14266 (-) | Length | 117 a.a. |
Antitoxin (Protein)
| Gene name | higA | Uniprot ID | - |
| Locus tag | CFNIH1_RS24455 | Protein ID | WP_000124640.1 |
| Coordinates | 13609..13911 (-) | Length | 101 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| CFNIH1_RS24405 | 9234..9662 | + | 429 | WP_000591074.1 | hypothetical protein | - |
| CFNIH1_RS24410 | 9719..10078 | + | 360 | WP_000422768.1 | hypothetical protein | - |
| CFNIH1_RS24415 | 10078..10524 | + | 447 | WP_000919345.1 | Fe3+-siderophore ABC transporter permease | - |
| CFNIH1_RS24420 | 10521..11039 | + | 519 | WP_000210756.1 | nitrite reductase | - |
| CFNIH1_RS24425 | 11039..11269 | + | 231 | WP_000972663.1 | hypothetical protein | - |
| CFNIH1_RS24430 | 11256..12113 | + | 858 | WP_001167032.1 | hypothetical protein | - |
| CFNIH1_RS24435 | 12139..12327 | + | 189 | WP_001270411.1 | hypothetical protein | - |
| CFNIH1_RS24440 | 12344..12871 | + | 528 | WP_001236377.1 | thermonuclease family protein | - |
| CFNIH1_RS24445 | 12929..13201 | + | 273 | WP_001043047.1 | HU family DNA-binding protein | - |
| CFNIH1_RS24450 | 13289..13582 | + | 294 | WP_001239997.1 | chromosome segregation protein ParM | - |
| CFNIH1_RS24455 | 13609..13911 | - | 303 | WP_000124640.1 | XRE family transcriptional regulator | Antitoxin |
| CFNIH1_RS24460 | 13916..14266 | - | 351 | WP_000270043.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| CFNIH1_RS24465 | 14429..14977 | + | 549 | WP_001061574.1 | transcriptional regulator | - |
| CFNIH1_RS27785 | 15318..15512 | + | 195 | WP_000343597.1 | hypothetical protein | - |
| CFNIH1_RS27790 | 15523..15894 | + | 372 | WP_000516916.1 | hypothetical protein | - |
| CFNIH1_RS24485 | 15887..16357 | + | 471 | WP_001281821.1 | hypothetical protein | - |
| CFNIH1_RS24490 | 16372..16707 | - | 336 | WP_000683476.1 | hypothetical protein | - |
| CFNIH1_RS24495 | 16804..17292 | + | 489 | WP_001273096.1 | DUF1643 domain-containing protein | - |
| CFNIH1_RS24500 | 17295..17792 | + | 498 | WP_000062185.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | blaFOX-5 / dfrA14 / ant(2'')-Ia / qacE / sul1 / msr(E) / mph(E) / blaKPC-2 / blaSHV-5 / aac(3)-Ia / qnrA1 | - | 1..272297 | 272297 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 117 a.a. Molecular weight: 13328.21 Da Isoelectric Point: 5.2421
>T46636 WP_000270043.1 NZ_CP007558:c14266-13916 [Citrobacter freundii CFNIH1]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE
Download Length: 351 bp
>T46636 NZ_CP007558:c14266-13916 [Citrobacter freundii CFNIH1]
ATGTGGGTCATCGAGACAACCGACACCTTTGATGAGTGGTTCGATGCTCTGGATGATACCGATAGAGCAAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGCCCCATGCTGTCGAGGCCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTTCGGGTCCAAAGCAAAGGAGATCCTATCAGAGCGTTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGTGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA
ATGTGGGTCATCGAGACAACCGACACCTTTGATGAGTGGTTCGATGCTCTGGATGATACCGATAGAGCAAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGCCCCATGCTGTCGAGGCCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTTCGGGTCCAAAGCAAAGGAGATCCTATCAGAGCGTTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGTGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA
Antitoxin
Download Length: 101 a.a. Molecular weight: 11006.73 Da Isoelectric Point: 7.2036
>AT46636 WP_000124640.1 NZ_CP007558:c13911-13609 [Citrobacter freundii CFNIH1]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV
Download Length: 303 bp
>AT46636 NZ_CP007558:c13911-13609 [Citrobacter freundii CFNIH1]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|