Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1679628..1680291 Replicon chromosome
Accession NZ_CP007557
Organism Citrobacter freundii CFNIH1

Toxin (Protein)


Gene name higB Uniprot ID A0A2Z3X092
Locus tag CFNIH1_RS08020 Protein ID WP_038636359.1
Coordinates 1679628..1679930 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A2Z3X2H4
Locus tag CFNIH1_RS08025 Protein ID WP_038636361.1
Coordinates 1679950..1680291 (+) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CFNIH1_RS08005 1675171..1676694 - 1524 WP_038636350.1 sugar ABC transporter ATP-binding protein -
CFNIH1_RS08010 1676809..1679073 + 2265 WP_038636353.1 hybrid sensor histidine kinase/response regulator -
CFNIH1_RS08015 1679143..1679472 + 330 WP_038636356.1 hypothetical protein -
CFNIH1_RS08020 1679628..1679930 + 303 WP_038636359.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CFNIH1_RS08025 1679950..1680291 + 342 WP_038636361.1 HigA family addiction module antidote protein Antitoxin
CFNIH1_RS08030 1680343..1681101 - 759 WP_038636364.1 phosphonate metabolism protein PhnP -
CFNIH1_RS08035 1681110..1681544 - 435 WP_038636367.1 aminoalkylphosphonate N-acetyltransferase -
CFNIH1_RS08040 1681531..1682085 - 555 WP_038636371.1 ribose 1,5-bisphosphokinase -
CFNIH1_RS08045 1682088..1683224 - 1137 WP_038636374.1 alpha-D-ribose 1-methylphosphonate 5-triphosphate diphosphatase -
CFNIH1_RS08050 1683221..1683901 - 681 WP_038636377.1 phosphonate C-P lyase system protein PhnL -
CFNIH1_RS08055 1684009..1684767 - 759 WP_038636380.1 phosphonate C-P lyase system protein PhnK -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Cro/C1-type helix-turn-helix domain

(23-70)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11856.50 Da        Isoelectric Point: 9.9581

>T46621 WP_038636359.1 NZ_CP007557:1679628-1679930 [Citrobacter freundii CFNIH1]
MSKSLNIRNFRDTWLEDFFERATPHRKIPADIHTALARKLDIINAAVSHRDLRSPPGNRYEELTGKLQEYSSIRVNKQYR
LIFKWVNGKAEDVYLDPHIY

Download         Length: 303 bp

>T46621 NZ_CP007557:1679628-1679930 [Citrobacter freundii CFNIH1]
ATGTCTAAATCATTAAATATCAGAAATTTTCGGGATACATGGCTTGAAGATTTTTTTGAGCGGGCAACACCGCACAGAAA
AATACCCGCGGATATTCACACCGCATTAGCCAGAAAGCTGGATATCATCAATGCGGCGGTTTCGCATCGGGATTTGCGCT
CGCCGCCAGGGAATAGATATGAAGAGTTAACCGGAAAGTTACAGGAATATTCTTCAATAAGAGTAAATAAGCAGTACCGA
TTAATATTTAAATGGGTTAACGGTAAGGCCGAAGATGTGTACTTAGACCCACACATCTACTAG

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13063.93 Da        Isoelectric Point: 7.2038

>AT46621 WP_038636361.1 NZ_CP007557:1679950-1680291 [Citrobacter freundii CFNIH1]
MKQATRKPTTVGDVLQYEYLEPLDLKINDLAEILHVHRNTVSALVNNNRKLTTDMAFRLAKAFDTSVDFWRNLQSAVDLW
EVENDMRAQEELNRIMSVKDFVAQRNVATKKVA

Download         Length: 342 bp

>AT46621 NZ_CP007557:1679950-1680291 [Citrobacter freundii CFNIH1]
ATGAAACAGGCAACCAGAAAACCAACTACTGTGGGTGATGTCCTGCAATATGAATACCTGGAGCCGCTCGATCTGAAAAT
TAACGATCTAGCTGAAATACTGCATGTTCACCGTAATACGGTGAGCGCACTGGTCAATAATAATCGCAAATTAACAACGG
ATATGGCATTTCGCCTGGCGAAAGCGTTTGATACCTCCGTTGATTTCTGGCGTAACCTACAGTCGGCGGTTGACCTGTGG
GAAGTTGAAAATGACATGCGGGCGCAAGAAGAACTGAACCGTATTATGTCTGTTAAAGACTTTGTCGCGCAGCGAAATGT
CGCCACTAAGAAAGTCGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2Z3X092

Antitoxin

Source ID Structure
AlphaFold DB A0A2Z3X2H4

References