Detailed information of TA system TA046615

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3873917..3874647
• Replicon: chromosome
• Accession: NZ_CP007546
• Organism: Enterobacter asburiae L1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A376FEI5
• Locus tag: DI57_RS18285
• Protein ID: WP_029741423.1
• Coordinates: 3874333..3874647 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: DI57_RS18280
• Protein ID: WP_029741422.1
• Coordinates: 3873917..3874336 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DI57_RS18260	3869389..3870894	-	1506	WP_029741419.1	signal transduction histidine-protein kinase/phosphatase UhpB	-
DI57_RS18265	3870891..3871484	-	594	WP_029741420.1	transcriptional regulator UhpA	-
DI57_RS18270	3871585..3871872	-	288	WP_010426528.1	acetolactate synthase small subunit	-
DI57_RS18275	3871876..3873564	-	1689	WP_029741421.1	acetolactate synthase large subunit	-
DI57_RS22690	3873671..3873769	-	99	WP_057979964.1	ilvB operon leader peptide IvbL	-
DI57_RS18280	3873917..3874336	-	420	WP_029741422.1	helix-turn-helix domain-containing protein	Antitoxin
DI57_RS18285	3874333..3874647	-	315	WP_029741423.1	type II toxin-antitoxin system HigB family toxin	Toxin
DI57_RS22280	3875134..3875223	+	90	WP_023309830.1	type I toxin-antitoxin system toxin TisB	-
DI57_RS18295	3875288..3875734	+	447	WP_029741424.1	GNAT family N-acetyltransferase	-
DI57_RS18300	3875797..3876630	+	834	WP_029741425.1	DMT family transporter	-
DI57_RS18305	3876806..3877990	+	1185	WP_029741426.1	multidrug efflux MFS transporter EmrD	-
DI57_RS18310	3877893..3878981	-	1089	WP_029741427.1	cellulase family glycosylhydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Antitoxin HigA (1-139)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 12361.46 Da
• Isoelectric Point: 10.2219

>T46615 WP_029741423.1 NZ_CP007546:c3874647-3874333 [Enterobacter asburiae L1]
MHLISMKAILDAVTQFPQHKDELLFLGRTIEKSHCPTPAALKKLFPTLDNFKYLDKHYVIDIANNNLRVVALIFFESQKF
YVRHVFTHKEYDRFTEKHRTKGKK

Nucleotide

• Length: 315 bp

>T46615 NZ_CP007546:c3874647-3874333 [Enterobacter asburiae L1]
ATGCATTTAATCTCTATGAAAGCTATTTTGGATGCGGTAACCCAGTTTCCGCAGCACAAGGATGAACTCCTTTTTTTGGG
TCGCACCATCGAAAAGAGCCATTGCCCAACACCTGCTGCTCTGAAAAAACTGTTTCCGACGCTGGATAACTTTAAGTATC
TGGATAAGCATTATGTTATTGATATTGCAAACAACAATCTCAGAGTAGTGGCTCTCATATTCTTTGAAAGCCAAAAGTTT
TATGTGCGCCATGTTTTTACTCATAAGGAATATGACCGTTTTACGGAGAAACATCGCACTAAGGGGAAGAAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15501.03 Da
• Isoelectric Point: 4.6363

>AT46615 WP_029741422.1 NZ_CP007546:c3874336-3873917 [Enterobacter asburiae L1]
MMIVADAMKATHALVAAVPLLGEQPSEKDYEDALELVEYLLMNEPDSPLLDIVCARISRYEANRPEIVALRKEMESVPVG
IAVLRTLMDQYNLTISDFQDEIGSKSMVSRVLNGQRQLTLNHIKKLAARFGVSPALFIE

Nucleotide

• Length: 420 bp

>AT46615 NZ_CP007546:c3874336-3873917 [Enterobacter asburiae L1]
ATGATGATCGTCGCTGATGCAATGAAAGCTACGCATGCCCTTGTTGCGGCCGTTCCTTTACTGGGTGAACAGCCGAGCGA
GAAAGATTATGAGGATGCGCTAGAGCTGGTTGAGTATCTTCTTATGAACGAGCCCGATAGCCCCTTGCTGGATATTGTGT
GCGCCAGGATTAGCCGTTACGAAGCCAATCGGCCAGAGATTGTCGCATTACGCAAGGAGATGGAGTCCGTTCCCGTCGGT
ATTGCGGTTTTAAGAACCTTGATGGATCAGTACAACCTGACGATATCGGATTTTCAGGATGAAATTGGCAGTAAATCGAT
GGTTTCACGGGTTCTGAATGGTCAGAGACAGCTTACTCTGAACCATATTAAAAAGCTGGCGGCGAGGTTTGGGGTATCAC
CTGCGCTGTTTATTGAGTGA

Structures

Toxin

• AlphaFold DB: A0A376FEI5