Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 1032466..1033126 Replicon chromosome
Accession NZ_CP007546
Organism Enterobacter asburiae L1

Toxin (Protein)


Gene name higB Uniprot ID A0A376FK36
Locus tag DI57_RS04860 Protein ID WP_029741461.1
Coordinates 1032466..1032819 (+) Length 118 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A1Z3MX79
Locus tag DI57_RS04865 Protein ID WP_029741460.1
Coordinates 1032824..1033126 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DI57_RS04840 1028608..1029525 + 918 WP_008499475.1 nitrogen assimilation transcriptional regulator NAC -
DI57_RS04845 1029622..1030572 + 951 WP_023336219.1 HTH-type transcriptional regulator Cbl -
DI57_RS04850 1030616..1031224 - 609 WP_038417400.1 glutathione S-transferase family protein -
DI57_RS04855 1031395..1032288 + 894 WP_029741462.1 LysR family transcriptional regulator -
DI57_RS04860 1032466..1032819 + 354 WP_029741461.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
DI57_RS04865 1032824..1033126 + 303 WP_029741460.1 XRE family transcriptional regulator Antitoxin
DI57_RS04870 1033202..1034122 - 921 WP_029741459.1 LysR family transcriptional regulator -
DI57_RS04875 1034264..1034665 + 402 WP_029741458.1 RidA family protein -
DI57_RS04885 1034960..1037149 - 2190 WP_029741691.1 TonB-dependent siderophore receptor -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1023937..1033126 9189


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-106)

Antitoxin

Cro/C1-type helix-turn-helix domain

(33-79)


Sequences

Toxin        


Download         Length: 118 a.a.        Molecular weight: 13531.36 Da        Isoelectric Point: 9.0310

>T46603 WP_029741461.1 NZ_CP007546:1032466-1032819 [Enterobacter asburiae L1]
VWAINTTDRFDRWFTSLEDTDRASVLAALLVLREKGPGLSRPYADTLKGSRHSNMKELRIQSKGDPLRAFFAFDPNRTGI
VLCAGNKVGNERRFYDEMLLVADREFTHWLNTLKERE

Download         Length: 354 bp

>T46603 NZ_CP007546:1032466-1032819 [Enterobacter asburiae L1]
GTGTGGGCAATTAACACGACAGACAGGTTTGATCGCTGGTTCACTTCGCTTGAGGATACCGATCGCGCCAGCGTACTTGC
AGCACTGCTTGTTTTGCGAGAGAAGGGGCCGGGTTTATCGAGACCCTACGCTGATACGCTTAAAGGCTCTCGTCACAGCA
ACATGAAAGAGCTACGTATTCAGAGTAAGGGCGATCCCTTGCGGGCATTTTTCGCCTTTGATCCAAACCGCACGGGTATC
GTGCTTTGTGCGGGCAATAAGGTCGGCAATGAAAGGCGATTCTACGATGAGATGCTTCTCGTTGCTGACCGGGAATTCAC
TCACTGGCTGAATACATTAAAAGAGAGGGAATAG

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11050.83 Da        Isoelectric Point: 5.7316

>AT46603 WP_029741460.1 NZ_CP007546:1032824-1033126 [Enterobacter asburiae L1]
MGRTLEQLIADEKPEVVASAQAIATDILLNIHLAELREKVQKTQVEMAQALGIRQPTVAVMEKPGRDLKLSSLKRYVEAA
GGKLRLDVELPDGSHYEFVL

Download         Length: 303 bp

>AT46603 NZ_CP007546:1032824-1033126 [Enterobacter asburiae L1]
ATGGGAAGAACTCTTGAGCAGCTTATAGCGGATGAAAAGCCGGAGGTTGTCGCCAGCGCTCAGGCGATAGCAACGGATAT
TTTGCTCAACATTCATCTTGCTGAGTTACGCGAGAAAGTACAAAAAACGCAGGTAGAAATGGCGCAGGCGTTGGGTATCA
GGCAACCTACCGTTGCGGTAATGGAAAAGCCCGGGCGCGATTTGAAACTCTCTTCTCTCAAGCGCTACGTTGAGGCGGCT
GGCGGAAAGCTGCGGCTTGATGTGGAGCTGCCGGATGGCTCGCACTATGAGTTTGTGCTGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A376FK36

Antitoxin

Source ID Structure
AlphaFold DB A0A1Z3MX79

References