Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/RelE-HTH
Location 3354829..3355439 Replicon chromosome
Accession NZ_CP007509
Organism Pseudomonas stutzeri strain 19SMN4

Toxin (Protein)


Gene name higB Uniprot ID A0A023WUP6
Locus tag UIB01_RS15455 Protein ID WP_038662341.1
Coordinates 3355125..3355439 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID I4CQU1
Locus tag UIB01_RS15450 Protein ID WP_003302338.1
Coordinates 3354829..3355119 (-) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
UIB01_RS15425 3350061..3351653 + 1593 WP_038662324.1 DNA cytosine methyltransferase -
UIB01_RS15430 3351650..3352114 + 465 WP_038662327.1 hypothetical protein -
UIB01_RS15435 3352111..3352422 + 312 WP_038662330.1 hypothetical protein -
UIB01_RS15440 3352409..3352609 + 201 WP_038662333.1 AlpA family phage regulatory protein -
UIB01_RS15445 3352613..3353812 - 1200 WP_038662336.1 integrase family protein -
UIB01_RS22870 3354113..3354655 - 543 WP_155268731.1 hypothetical protein -
UIB01_RS15450 3354829..3355119 - 291 WP_003302338.1 helix-turn-helix domain-containing protein Antitoxin
UIB01_RS15455 3355125..3355439 - 315 WP_038662341.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
UIB01_RS15460 3355484..3356509 - 1026 WP_038662345.1 tyrosine-type recombinase/integrase -
UIB01_RS15465 3356491..3357768 - 1278 WP_038662348.1 hypothetical protein -
UIB01_RS15470 3357916..3359100 - 1185 WP_038662351.1 zonular occludens toxin -
UIB01_RS15475 3359113..3359439 - 327 WP_038662353.1 DUF2523 domain-containing protein -
UIB01_RS15480 3359439..3359756 - 318 WP_038665873.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 3314532..3368223 53691


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-2

(43-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(35-86)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11923.68 Da        Isoelectric Point: 9.8035

>T46570 WP_038662341.1 NZ_CP007509:c3355439-3355125 [Pseudomonas stutzeri]
MIFIETPIFSKRLRELLSDDSYAEFQRQLADRPDMGDVIEGTGGIRKVRVASGGHGKRGGSRVIYYHFTAASQIALLLIY
PKNEKDDLTADERKVLKQIIERWR

Download         Length: 315 bp

>T46570 NZ_CP007509:c3355439-3355125 [Pseudomonas stutzeri]
ATGATCTTTATCGAGACACCGATCTTCTCCAAGCGTCTGCGGGAGCTGCTTAGCGATGACAGCTATGCGGAGTTTCAGCG
GCAACTGGCCGACCGGCCGGACATGGGCGATGTGATTGAAGGTACTGGCGGCATTCGCAAGGTTCGCGTTGCGTCTGGTG
GTCACGGCAAGCGGGGCGGGTCCAGGGTCATCTACTACCACTTCACGGCGGCTTCGCAGATCGCATTACTGCTGATCTAT
CCGAAGAACGAGAAGGACGACCTGACGGCAGACGAGCGCAAGGTGCTCAAGCAAATCATCGAACGGTGGAGGTAA

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10925.51 Da        Isoelectric Point: 8.8068

>AT46570 WP_003302338.1 NZ_CP007509:c3355119-3354829 [Pseudomonas stutzeri]
MSKFFEDLLESVQQMDEIHRGERQPSREFVVDSLQVKEIRKATGLTQAKFAAMIDVQLGTLRNWEQGRREPTGPAKALLR
AIHNDPKHVIQALSSV

Download         Length: 291 bp

>AT46570 NZ_CP007509:c3355119-3354829 [Pseudomonas stutzeri]
ATGAGCAAATTCTTCGAAGACCTTCTGGAAAGCGTCCAGCAGATGGACGAGATTCACCGTGGCGAACGTCAGCCCTCGCG
GGAATTCGTCGTCGACTCGCTGCAGGTGAAAGAGATCCGCAAGGCGACCGGGCTGACCCAGGCCAAATTCGCAGCGATGA
TCGACGTTCAACTGGGTACGCTGCGTAACTGGGAACAAGGCCGACGCGAACCGACCGGACCAGCAAAGGCCCTGCTGCGC
GCCATCCATAACGATCCGAAGCACGTCATCCAGGCGCTATCGTCCGTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A023WUP6

Antitoxin

Source ID Structure
AlphaFold DB I4CQU1

References