Detailed information of TA system TA046564

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1490603..1491169
• Replicon: chromosome
• Accession: NZ_CP007502
• Organism: Aggregatibacter actinomycetemcomitans HK1651

Toxin (Protein)

• Gene name: higB
• Uniprot ID: X2JU35
• Locus tag: CF65_RS07270
• Protein ID: WP_005543444.1
• Coordinates: 1490603..1490881 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: CF65_RS07275
• Protein ID: WP_005543446.1
• Coordinates: 1490897..1491169 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CF65_RS07245	1486159..1486713	+	555	WP_005543432.1	HAD family hydrolase	-
CF65_RS07250	1486881..1488428	+	1548	WP_025298400.1	anthranilate synthase component 1	-
CF65_RS07255	1488439..1489029	+	591	WP_005543437.1	aminodeoxychorismate/anthranilate synthase component II	-
CF65_RS07260	1489103..1489495	+	393	WP_005543439.1	tautomerase family protein	-
CF65_RS07265	1489507..1490508	+	1002	WP_005567382.1	anthranilate phosphoribosyltransferase	-
CF65_RS07270	1490603..1490881	+	279	WP_005543444.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CF65_RS07275	1490897..1491169	+	273	WP_005543446.1	HigA family addiction module antidote protein	Antitoxin
CF65_RS07280	1491178..1492604	+	1427	Protein_1437	bifunctional indole-3-glycerol-phosphate synthase TrpC/phosphoribosylanthranilate isomerase TrpF	-
CF65_RS07285	1493059..1493334	+	276	WP_005561117.1	hydrogenase maturation factor HybG	-
CF65_RS07290	1493455..1493832	-	378	WP_005543455.1	hypothetical protein	-
CF65_RS10625	1493807..1493998	-	192	WP_005553960.1	hypothetical protein	-
CF65_RS07295	1494359..1494625	+	267	WP_005543457.1	oxaloacetate decarboxylase subunit gamma	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-79)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10490.95 Da
• Isoelectric Point: 9.5983

>T46564 WP_005543444.1 NZ_CP007502:1490603-1490881 [Aggregatibacter actinomycetemcomitans HK1651]
MILSFKHKGLEQFFKTGSTAGIQAKHAKKLNLQLATLNNAETPLAMSVPSWNLHKLKGDLQEHWAVTVNGNWRLTFKFEN
GHAEVVDYQDYH

Nucleotide

• Length: 279 bp

>T46564 NZ_CP007502:1490603-1490881 [Aggregatibacter actinomycetemcomitans HK1651]
ATGATTTTATCTTTCAAACATAAGGGACTTGAACAATTTTTTAAAACCGGTTCGACCGCAGGAATTCAAGCCAAACACGC
CAAGAAATTAAATCTGCAATTGGCCACATTAAATAATGCGGAAACGCCTCTTGCTATGAGTGTTCCCAGCTGGAATTTGC
ACAAACTTAAAGGTGATTTGCAAGAGCATTGGGCAGTTACCGTAAACGGAAATTGGCGCTTAACATTTAAATTTGAAAAT
GGTCATGCTGAAGTTGTTGATTATCAGGATTATCATTAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10138.74 Da
• Isoelectric Point: 7.3169

>AT46564 WP_005543446.1 NZ_CP007502:1490897-1491169 [Aggregatibacter actinomycetemcomitans HK1651]
MHNPPHPGELLKEYIDGVSVTKVAQKLGVSRVTLSRILNGKASITPEMAVRLSELLNTTTPKLWLGMQADYDLWQIEQQH
AVFNIQPLFN

Nucleotide

• Length: 273 bp

>AT46564 NZ_CP007502:1490897-1491169 [Aggregatibacter actinomycetemcomitans HK1651]
ATGCACAATCCTCCACACCCGGGAGAACTGTTAAAAGAATATATTGATGGCGTGAGTGTCACGAAGGTTGCCCAAAAATT
AGGTGTTTCGCGTGTTACGCTTTCCCGCATTCTTAATGGGAAAGCAAGTATAACGCCTGAAATGGCTGTGCGATTAAGCG
AATTATTGAATACCACAACACCGAAATTATGGCTGGGTATGCAAGCAGACTACGATTTATGGCAAATTGAACAACAACAC
GCCGTATTCAACATCCAACCATTATTTAATTAA

Structures

Toxin

• AlphaFold DB: X2JU35