Detailed information of TA system TA046562

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 1246802..1247448
• Replicon: chromosome
• Accession: NZ_CP007502
• Organism: Aggregatibacter actinomycetemcomitans HK1651

Toxin (Protein)

• Gene name: higB
• Uniprot ID: X2JQ23
• Locus tag: CF65_RS06200
• Protein ID: WP_005555126.1
• Coordinates: 1246802..1247152 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: X2JT85
• Locus tag: CF65_RS06205
• Protein ID: WP_005540827.1
• Coordinates: 1247149..1247448 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CF65_RS06180	1241980..1242225	-	246	WP_005540815.1	YgjV family protein	-
CF65_RS06185	1242415..1243752	-	1338	WP_005567415.1	argininosuccinate synthase	-
CF65_RS06190	1243847..1244674	-	828	WP_005540818.1	hypothetical protein	-
CF65_RS06195	1245031..1246614	+	1584	WP_005567417.1	peptide chain release factor 3	-
CF65_RS06200	1246802..1247152	+	351	WP_005555126.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CF65_RS06205	1247149..1247448	+	300	WP_005540827.1	helix-turn-helix transcriptional regulator	Antitoxin
CF65_RS06210	1247484..1250417	-	2934	WP_025298333.1	bifunctional [glutamate--ammonia ligase]-adenylyl-L-tyrosine phosphorylase/[glutamate--ammonia-ligase] adenylyltransferase	-
CF65_RS06215	1250619..1250846	+	228	WP_005567423.1	hypothetical protein	-
CF65_RS06220	1250839..1251567	+	729	Protein_1222	CRISPR-associated endonuclease Cas3''	-
CF65_RS06225	1251946..1252346	+	401	Protein_1223	bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-80)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13518.64 Da
• Isoelectric Point: 9.9664

>T46562 WP_005555126.1 NZ_CP007502:1246802-1247152 [Aggregatibacter actinomycetemcomitans HK1651]
MWAVILTERFETWLNQQPIKTQECVLANLVRLEHFGPNLARPYVDSVAGSIHSNMKELRVQHSGRAIRLFFAFDPKRRAI
VLCGGDKGNDKRFYRTMIRIADQELNHYLATMEQTK

Nucleotide

• Length: 351 bp

>T46562 NZ_CP007502:1246802-1247152 [Aggregatibacter actinomycetemcomitans HK1651]
ATGTGGGCGGTGATTTTAACGGAACGATTTGAAACTTGGTTAAATCAACAGCCGATAAAAACGCAGGAATGTGTTTTGGC
AAACTTGGTACGGTTGGAACATTTTGGTCCGAATTTAGCCCGTCCTTATGTAGATAGCGTAGCAGGGTCAATTCATAGCA
ACATGAAAGAACTACGCGTTCAGCATTCGGGAAGAGCAATTCGACTCTTTTTTGCTTTTGATCCAAAGCGTAGAGCCATT
GTTTTATGTGGTGGAGATAAAGGCAATGACAAGCGGTTTTACCGTACGATGATTAGAATTGCAGATCAAGAATTGAATCA
TTATTTGGCAACCATGGAGCAAACAAAATGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11342.12 Da
• Isoelectric Point: 5.8598

>AT46562 WP_005540827.1 NZ_CP007502:1247149-1247448 [Aggregatibacter actinomycetemcomitans HK1651]
MKTLQQVIDERSVESRQRIQTMAEDLILETGLQLLREELNISQQQLATILGVTQPAINQLEQRGNEIKLATLKRYIEAMG
GKLRLSVEMPDGHQRVFQI

Nucleotide

• Length: 300 bp

>AT46562 NZ_CP007502:1247149-1247448 [Aggregatibacter actinomycetemcomitans HK1651]
ATGAAAACATTACAACAAGTTATTGATGAACGTTCGGTAGAAAGCCGCCAACGTATTCAAACCATGGCGGAAGATTTAAT
TCTTGAAACCGGTTTGCAACTGTTGCGAGAAGAATTAAATATATCGCAACAACAATTAGCTACGATTTTAGGTGTAACTC
AACCTGCAATTAATCAACTGGAACAGCGTGGCAATGAAATTAAGCTTGCAACCCTCAAACGTTACATCGAAGCAATGGGC
GGCAAATTACGCCTTAGTGTAGAAATGCCTGATGGACATCAGCGGGTTTTTCAAATTTAA

Structures

Toxin

• AlphaFold DB: X2JQ23

Antitoxin

• AlphaFold DB: X2JT85