Detailed information of TA system TA046520

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2887394..2888121
• Replicon: chromosome
• Accession: NZ_CP007491
• Organism: Escherichia coli strain ACN002

Toxin (Protein)

• Gene name: higB
• Uniprot ID: J7Q991
• Locus tag: ACN002_RS14185
• Protein ID: WP_000547564.1
• Coordinates: 2887810..2888121 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ACN002_RS14180
• Protein ID: WP_000126294.1
• Coordinates: 2887394..2887813 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ACN002_RS14160	2882444..2882971	-	528	WP_001078777.1	electron transport protein HydN	-
ACN002_RS14165	2883120..2884130	-	1011	WP_001392554.1	DNA-binding transcriptional regulator AscG	-
ACN002_RS14170	2884390..2885847	+	1458	WP_001107861.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
ACN002_RS14175	2885856..2887280	+	1425	WP_000110363.1	6-phospho-beta-glucosidase AscB	-
ACN002_RS14180	2887394..2887813	-	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
ACN002_RS14185	2887810..2888121	-	312	WP_000547564.1	type II toxin-antitoxin system HigB family toxin	Toxin
ACN002_RS14190	2888295..2889056	-	762	WP_001026446.1	hypothetical protein	-
ACN002_RS14195	2889081..2889551	-	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
ACN002_RS14200	2889544..2889954	-	411	WP_001291921.1	formate hydrogenlyase assembly protein HycH	-
ACN002_RS14205	2889951..2890718	-	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
ACN002_RS14210	2890718..2891260	-	543	WP_000493792.1	formate hydrogenlyase subunit HycF	-
ACN002_RS14215	2891270..2892979	-	1710	WP_001288134.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12426.13 Da
• Isoelectric Point: 9.7248

>T46520 WP_000547564.1 NZ_CP007491:c2888121-2887810 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T46520 NZ_CP007491:c2888121-2887810 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAGATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT46520 WP_000126294.1 NZ_CP007491:c2887813-2887394 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT46520 NZ_CP007491:c2887813-2887394 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAGTATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCGGTT
GGCGTGGCGTTACTTCGCACGTTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A0A0FJG5