Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2171171..2171673 Replicon chromosome
Accession NZ_CP007491
Organism Escherichia coli strain ACN002

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag ACN002_RS10810 Protein ID WP_000767829.1
Coordinates 2171171..2171425 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag ACN002_RS10815 Protein ID WP_001259255.1
Coordinates 2171422..2171673 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ACN002_RS10785 2166186..2166413 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
ACN002_RS10790 2166427..2167485 - 1059 WP_000492346.1 transport protein YeeE -
ACN002_RS10795 2167664..2169022 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
ACN002_RS26700 2169012..2169074 - 63 WP_010723108.1 membrane protein YoeI -
ACN002_RS10800 2169289..2170218 - 930 WP_000803351.1 LysR family transcriptional regulator -
ACN002_RS10805 2170264..2171088 - 825 WP_000754758.1 SDR family oxidoreductase -
ACN002_RS10810 2171171..2171425 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
ACN002_RS10815 2171422..2171673 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
ACN002_RS25005 2171957..2172007 + 51 WP_001364200.1 his operon leader peptide -
ACN002_RS10820 2172153..2173052 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
ACN002_RS10825 2173058..2174362 + 1305 WP_001317939.1 histidinol dehydrogenase -
ACN002_RS10830 2174359..2175429 + 1071 WP_000108941.1 histidinol-phosphate transaminase -
ACN002_RS10835 2175429..2176496 + 1068 WP_000080076.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T46518 WP_000767829.1 NZ_CP007491:c2171425-2171171 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T46518 NZ_CP007491:c2171425-2171171 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT46518 WP_001259255.1 NZ_CP007491:c2171673-2171422 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT46518 NZ_CP007491:c2171673-2171422 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATATAACTCCCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References