Detailed information of TA system TA046498

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1439047..1439686
• Replicon: chromosome
• Accession: NZ_CP007471
• Organism: Haemophilus influenzae strain C486

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q4QJX7
• Locus tag: NTHIC486_RS07000
• Protein ID: WP_005650215.1
• Coordinates: 1439381..1439686 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: NTHIC486_RS06995
• Protein ID: WP_005650217.1
• Coordinates: 1439047..1439370 (-)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NTHIC486_RS09300	1434666..1434839	-	174	WP_011272700.1	DUF5363 domain-containing protein	-
NTHIC486_RS06980	1434836..1436806	-	1971	WP_044345713.1	tRNA(Met) cytidine acetyltransferase TmcA	-
NTHIC486_RS06985	1436811..1437152	-	342	WP_044345715.1	SirB2 family protein	-
NTHIC486_RS06990	1437214..1438884	-	1671	WP_044345717.1	energy-dependent translational throttle protein EttA	-
NTHIC486_RS06995	1439047..1439370	-	324	WP_005650217.1	HigA family addiction module antidote protein	Antitoxin
NTHIC486_RS07000	1439381..1439686	-	306	WP_005650215.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
NTHIC486_RS07005	1440011..1440631	+	621	WP_005666732.1	zinc transporter binding subunit ZevA	-
NTHIC486_RS07010	1440634..1441602	+	969	WP_005666730.1	zinc transporter permease subunit ZevB	-
NTHIC486_RS07020	1442218..1444257	+	2040	WP_005687320.1	excinuclease ABC subunit B	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-99)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (4-82)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 12301.93 Da
• Isoelectric Point: 9.0853

>T46498 WP_005650215.1 NZ_CP007471:c1439686-1439381 [Haemophilus influenzae]
MFNLKREHFRDDYLYRFYQYGDTHSKIPSNLYKVLARKLDMISASENINDLRSPPANHLELLEPKENKIYSIRVNKQYRL
IFKYENNEVNNLYLDPHSYNL

Nucleotide

• Length: 306 bp

>T46498 NZ_CP007471:c1439686-1439381 [Haemophilus influenzae]
ATGTTTAATTTAAAGCGTGAGCATTTTAGAGATGATTATCTTTATAGGTTTTATCAATATGGTGATACTCATAGTAAGAT
TCCTTCTAATTTGTATAAAGTATTGGCAAGAAAATTAGATATGATAAGTGCCTCAGAAAATATCAACGATTTACGTTCTC
CACCAGCTAACCATTTGGAGTTGCTTGAGCCAAAAGAAAATAAAATTTATTCTATTCGAGTGAATAAACAATATCGTTTA
ATTTTTAAATATGAAAATAACGAAGTAAATAATTTATATTTAGATCCACATTCTTATAATTTGTAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 12347.17 Da
• Isoelectric Point: 6.8804

>AT46498 WP_005650217.1 NZ_CP007471:c1439370-1439047 [Haemophilus influenzae]
MMTRKPTSVGEILQEEFLEPLSLKISDLAQILDVHRNTASNIVNNSSRITLEMAVKLAKVFDTTPEFWLNLQTRIDLWDL
EHNKRFQQSLANVKPALHRHDTSTFAM

Nucleotide

• Length: 324 bp

>AT46498 NZ_CP007471:c1439370-1439047 [Haemophilus influenzae]
ATGATGACGAGAAAACCCACCTCTGTTGGTGAAATTCTACAAGAAGAGTTTTTAGAACCTTTAAGTCTTAAAATTAGTGA
TTTAGCTCAAATTTTAGATGTACATCGTAATACGGCAAGCAATATTGTTAATAATAGTTCACGAATTACGCTAGAAATGG
CAGTTAAACTTGCTAAAGTATTTGATACTACGCCTGAGTTTTGGCTTAATCTGCAAACACGTATTGATTTATGGGACCTT
GAACACAATAAACGTTTTCAACAAAGTCTTGCAAATGTTAAGCCCGCTCTACATCGGCATGATACTTCTACATTTGCAAT
GTAA

Structures

Toxin

• AlphaFold DB: A0A806EC70