Detailed information of TA system TA046485

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 400201..400840
• Replicon: chromosome
• Accession: NZ_CP007470
• Organism: Haemophilus influenzae strain 477

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q4QJX7
• Locus tag: NTHI477_RS01950
• Protein ID: WP_005650215.1
• Coordinates: 400535..400840 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: NTHI477_RS01945
• Protein ID: WP_005650217.1
• Coordinates: 400201..400524 (-)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NTHI477_RS09435	395820..395993	-	174	WP_172644278.1	DUF5363 family protein	-
NTHI477_RS01930	395990..397960	-	1971	WP_044330238.1	tRNA(Met) cytidine acetyltransferase TmcA	-
NTHI477_RS01935	397965..398306	-	342	WP_044330240.1	SirB2 family protein	-
NTHI477_RS01940	398368..400038	-	1671	WP_005650218.1	energy-dependent translational throttle protein EttA	-
NTHI477_RS01945	400201..400524	-	324	WP_005650217.1	HigA family addiction module antidote protein	Antitoxin
NTHI477_RS01950	400535..400840	-	306	WP_005650215.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
NTHI477_RS01955	401164..401784	+	621	WP_005694310.1	zinc transporter binding subunit ZevA	-
NTHI477_RS01960	401787..402755	+	969	WP_042593950.1	zinc transporter permease subunit ZevB	-
NTHI477_RS01970	403370..405409	+	2040	WP_038441065.1	excinuclease ABC subunit B	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-99)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (4-82)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 12301.93 Da
• Isoelectric Point: 9.0853

>T46485 WP_005650215.1 NZ_CP007470:c400840-400535 [Haemophilus influenzae]
MFNLKREHFRDDYLYRFYQYGDTHSKIPSNLYKVLARKLDMISASENINDLRSPPANHLELLEPKENKIYSIRVNKQYRL
IFKYENNEVNNLYLDPHSYNL

Nucleotide

• Length: 306 bp

>T46485 NZ_CP007470:c400840-400535 [Haemophilus influenzae]
ATGTTTAATTTAAAGCGTGAGCATTTTAGAGATGATTATCTTTATAGGTTTTATCAATATGGTGATACTCATAGTAAGAT
TCCTTCTAATTTGTATAAAGTATTGGCAAGAAAATTAGATATGATAAGTGCCTCAGAAAATATCAACGATTTACGTTCTC
CACCAGCTAACCATTTGGAGTTGCTTGAGCCAAAAGAAAATAAAATTTATTCTATTCGAGTGAATAAACAATATCGTTTA
ATTTTTAAATATGAAAATAACGAAGTAAATAATTTATATTTAGATCCACATTCTTATAATTTGTAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 12347.17 Da
• Isoelectric Point: 6.8804

>AT46485 WP_005650217.1 NZ_CP007470:c400524-400201 [Haemophilus influenzae]
MMTRKPTSVGEILQEEFLEPLSLKISDLAQILDVHRNTASNIVNNSSRITLEMAVKLAKVFDTTPEFWLNLQTRIDLWDL
EHNKRFQQSLANVKPALHRHDTSTFAM

Nucleotide

• Length: 324 bp

>AT46485 NZ_CP007470:c400524-400201 [Haemophilus influenzae]
ATGATGACGAGAAAACCCACCTCTGTTGGTGAAATTCTACAAGAAGAGTTTTTAGAACCTTTAAGTCTTAAAATTAGTGA
TTTAGCTCAAATTTTAGATGTACATCGTAATACGGCAAGCAATATTGTTAATAATAGTTCACGAATTACGCTAGAAATGG
CAGTTAAACTTGCTAAAGTATTTGATACTACGCCTGAGTTTTGGCTTAATCTGCAAACACGTATTGATTTATGGGACCTT
GAACACAATAAACGTTTTCAACAAAGTCTTGCAAATGTTAAGCCCGCTCTACATCGGCATGATACTTCTACATTTGCAAT
GTAA

Structures

Toxin

• AlphaFold DB: A0A806EC70