Detailed information of TA system TA046436

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2913239..2913966
• Replicon: chromosome
• Accession: NZ_CP007442
• Organism: Escherichia coli ACN001

Toxin (Protein)

• Gene name: higB
• Uniprot ID: J7Q991
• Locus tag: J444_RS14305
• Protein ID: WP_000547564.1
• Coordinates: 2913655..2913966 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: J444_RS14300
• Protein ID: WP_000126294.1
• Coordinates: 2913239..2913658 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J444_RS14280	2908289..2908816	-	528	WP_001078777.1	electron transport protein HydN	-
J444_RS14285	2908965..2909975	-	1011	WP_001392554.1	DNA-binding transcriptional regulator AscG	-
J444_RS14290	2910235..2911692	+	1458	WP_001107861.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
J444_RS14295	2911701..2913125	+	1425	WP_000110363.1	6-phospho-beta-glucosidase AscB	-
J444_RS14300	2913239..2913658	-	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
J444_RS14305	2913655..2913966	-	312	WP_000547564.1	type II toxin-antitoxin system HigB family toxin	Toxin
J444_RS14310	2914140..2914901	-	762	WP_001026446.1	hypothetical protein	-
J444_RS14315	2914926..2915396	-	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
J444_RS14320	2915389..2915799	-	411	WP_001291921.1	formate hydrogenlyase assembly protein HycH	-
J444_RS14325	2915796..2916563	-	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
J444_RS14330	2916563..2917105	-	543	WP_000493792.1	formate hydrogenlyase subunit HycF	-
J444_RS14335	2917115..2918824	-	1710	WP_001288134.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12426.13 Da
• Isoelectric Point: 9.7248

>T46436 WP_000547564.1 NZ_CP007442:c2913966-2913655 [Escherichia coli ACN001]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T46436 NZ_CP007442:c2913966-2913655 [Escherichia coli ACN001]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAGATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT46436 WP_000126294.1 NZ_CP007442:c2913658-2913239 [Escherichia coli ACN001]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT46436 NZ_CP007442:c2913658-2913239 [Escherichia coli ACN001]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAGTATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCGGTT
GGCGTGGCGTTACTTCGCACGTTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A0A0FJG5