Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2116928..2117430 Replicon chromosome
Accession NZ_CP007442
Organism Escherichia coli ACN001

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag J444_RS10360 Protein ID WP_000767829.1
Coordinates 2116928..2117182 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag J444_RS10365 Protein ID WP_001259255.1
Coordinates 2117179..2117430 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J444_RS10335 2111943..2112170 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
J444_RS10340 2112184..2113242 - 1059 WP_000492346.1 transport protein YeeE -
J444_RS10345 2113421..2114779 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
J444_RS27035 2114769..2114831 - 63 WP_010723108.1 membrane protein YoeI -
J444_RS10350 2115046..2115975 - 930 WP_000803351.1 LysR family transcriptional regulator -
J444_RS10355 2116021..2116845 - 825 WP_000754758.1 SDR family oxidoreductase -
J444_RS10360 2116928..2117182 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
J444_RS10365 2117179..2117430 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
J444_RS25275 2117714..2117764 + 51 WP_001364200.1 his operon leader peptide -
J444_RS10370 2117910..2118809 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
J444_RS10375 2118815..2120119 + 1305 WP_001317939.1 histidinol dehydrogenase -
J444_RS10380 2120116..2121186 + 1071 WP_000108941.1 histidinol-phosphate transaminase -
J444_RS10385 2121186..2122253 + 1068 WP_000080076.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T46434 WP_000767829.1 NZ_CP007442:c2117182-2116928 [Escherichia coli ACN001]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T46434 NZ_CP007442:c2117182-2116928 [Escherichia coli ACN001]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT46434 WP_001259255.1 NZ_CP007442:c2117430-2117179 [Escherichia coli ACN001]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT46434 NZ_CP007442:c2117430-2117179 [Escherichia coli ACN001]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATATAACTCCCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References