Detailed information of TA system TA046416

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 4642720..4643276
• Replicon: chromosome
• Accession: NZ_CP007410
• Organism: Pseudomonas brassicacearum strain DF41

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: CD58_RS19715
• Protein ID: WP_049866994.1
• Coordinates: 4642720..4642986 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CD58_RS19720
• Protein ID: WP_025214709.1
• Coordinates: 4642989..4643276 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CD58_RS19700	4638887..4640458	-	1572	WP_025214705.1	diguanylate cyclase	-
CD58_RS19705	4640758..4642116	+	1359	WP_025214706.1	chromate efflux transporter	-
CD58_RS19710	4642113..4642544	-	432	WP_025214707.1	GNAT family N-acetyltransferase	-
CD58_RS19715	4642720..4642986	+	267	WP_049866994.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CD58_RS19720	4642989..4643276	+	288	WP_025214709.1	helix-turn-helix domain-containing protein	Antitoxin
CD58_RS19725	4643410..4643898	+	489	WP_025214710.1	hypothetical protein	-
CD58_RS19730	4643931..4644956	-	1026	WP_025214711.1	DeoR family transcriptional regulator	-
CD58_RS19735	4645140..4646291	+	1152	WP_025214712.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (27-86)

Antitoxin

• Cro/C1-type helix-turn-helix domain (35-87)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 9968.52 Da
• Isoelectric Point: 10.2225

>T46416 WP_049866994.1 NZ_CP007410:4642720-4642986 [Pseudomonas brassicacearum]
MNDDDFSALQNILVRNPSAGDVMEATSGIRKIRIAAKGHGKRGGARVIYYHFVSASQIALLMIYPKNEQQDLTVDQRKAL
KMIIEHWR

Nucleotide

• Length: 267 bp

>T46416 NZ_CP007410:4642720-4642986 [Pseudomonas brassicacearum]
ATGAATGACGACGACTTTTCAGCCCTGCAGAACATATTGGTCCGCAACCCTTCGGCTGGCGATGTAATGGAAGCCACCAG
TGGGATTCGAAAGATCCGTATCGCGGCAAAGGGCCACGGCAAACGGGGCGGGGCCAGGGTCATTTATTACCACTTTGTGT
CTGCTTCGCAGATCGCATTATTGATGATATATCCAAAAAACGAACAGCAAGACTTGACCGTTGATCAACGCAAAGCATTG
AAAATGATCATCGAGCACTGGAGATAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10811.40 Da
• Isoelectric Point: 6.4713

>AT46416 WP_025214709.1 NZ_CP007410:4642989-4643276 [Pseudomonas brassicacearum]
MSRFFDELMESVQQMDEIIRGERQPSREFIVDALQVKEIRKATGLTQAKFAALIDVQLGTLRNWEQGRREPTGPAKALLR
AIHNDPEHVINALAV

Nucleotide

• Length: 288 bp

>AT46416 NZ_CP007410:4642989-4643276 [Pseudomonas brassicacearum]
ATGAGCAGATTTTTCGATGAGTTGATGGAAAGCGTGCAGCAGATGGACGAAATTATCCGAGGCGAGCGTCAGCCTTCCCG
AGAGTTCATCGTCGATGCGTTACAAGTGAAGGAAATACGCAAGGCTACCGGCCTGACTCAAGCCAAGTTTGCAGCATTGA
TCGACGTCCAACTGGGCACGCTTCGCAACTGGGAACAAGGACGACGTGAGCCAACAGGCCCCGCGAAAGCCCTCTTGCGG
GCAATTCATAATGATCCTGAACATGTGATCAACGCCTTGGCGGTTTGA