Detailed information of TA system TA046407

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 1499598..1500193
• Replicon: chromosome
• Accession: NZ_CP007399
• Organism: Pseudomonas aeruginosa strain F22031

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: F22031_RS30655
• Protein ID: WP_003113526.1
• Coordinates: 1499915..1500193 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: F22031_RS06950
• Protein ID: WP_003099268.1
• Coordinates: 1499598..1499903 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F22031_RS31610	1496328..1497017	-	690	WP_128725157.1	hypothetical protein	-
F22031_RS31615	1497014..1498291	-	1278	WP_128725156.1	hypothetical protein	-
F22031_RS31620	1498432..1499091	-	660	WP_128725155.1	hypothetical protein	-
F22031_RS06950	1499598..1499903	-	306	WP_003099268.1	HigA family addiction module antidote protein	Antitoxin
F22031_RS30655	1499915..1500193	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
F22031_RS06955	1500522..1502750	+	2229	WP_003099265.1	TonB-dependent receptor	-
F22031_RS06960	1502820..1503467	-	648	WP_003095021.1	carbonate dehydratase	-
F22031_RS06965	1503529..1504767	-	1239	WP_019681675.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-97)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T46407 WP_003113526.1 NZ_CP007399:c1500193-1499915 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T46407 NZ_CP007399:c1500193-1499915 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11213.79 Da
• Isoelectric Point: 5.0747

>AT46407 WP_003099268.1 NZ_CP007399:c1499903-1499598 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT46407 NZ_CP007399:c1499903-1499598 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCCGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: V6ALY3