Detailed information of TA system TA046386

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 2099578..2099949
• Replicon: chromosome
• Accession: NZ_CP007394
• Organism: Escherichia coli strain ST2747

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: A0A3K0WCG2
• Locus tag: CF60_RS10125
• Protein ID: WP_032159264.1
• Coordinates: 2099755..2099949 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	2099578..2099756 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CF60_RS10100	2095533..2096906	+	1374	WP_001599773.1	ATP-dependent RNA helicase DbpA	-
CF60_RS10105	2097035..2097970	-	936	WP_001157407.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
CF60_RS25795	2098022..2099257	-	1236	WP_001516465.1	site-specific integrase	-
CF60_RS10115	2099259..2099474	-	216	WP_000079604.1	excisionase XisR	-
CF60_RS10120	2099574..2099762	-	189	WP_001605349.1	DUF1187 family protein	-
-	2099578..2099756	+	179	NuclAT_0	-	Antitoxin
-	2099578..2099756	+	179	NuclAT_0	-	Antitoxin
-	2099578..2099756	+	179	NuclAT_0	-	Antitoxin
-	2099578..2099756	+	179	NuclAT_0	-	Antitoxin
CF60_RS10125	2099755..2099949	-	195	WP_032159264.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
CF60_RS10130	2100013..2101065	-	1053	WP_001004422.1	recombinase RecT	-
CF60_RS10135	2101077..2104148	-	3072	WP_001605350.1	exodeoxyribonuclease VIII	-
CF60_RS10140	2104250..2104525	-	276	WP_001344816.1	hypothetical protein	-
CF60_RS10145	2104600..2104770	-	171	WP_001352098.1	conserved protein, Rac prophage	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2098022..2192917	94895

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7063.03 Da
• Isoelectric Point: 9.2886

>T46386 WP_032159264.1 NZ_CP007394:c2099949-2099755 [Escherichia coli]
MRYEKVKPCPFCGCLSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T46386 NZ_CP007394:c2099949-2099755 [Escherichia coli]
ATGCGGTATGAAAAAGTCAAACCATGCCCTTTTTGTGGTTGTCTATCAGTAACGGTAAAAGCCATTTCAGGATATTACCG
CGCGAAGTGTAACGGATGCGAATCCCGAACCGGCTATGGTGGAAGTGAAAAAGAAGCACTCGAACGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT46386 NZ_CP007394:2099578-2099756 [Escherichia coli]
GACGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAACCGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTGGTTAATTTTGTTTTTGAGTACCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A3K0WCG2

Antitoxin