Detailed information of TA system TA046350

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 917498..918192
• Replicon: chromosome
• Accession: NZ_CP007393
• Organism: Escherichia coli strain ST2747

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: -
• Locus tag: CF59_RS04350
• Protein ID: WP_025210215.1
• Coordinates: 917794..918192 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: CF59_RS04345
• Protein ID: WP_000554758.1
• Coordinates: 917498..917791 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CF59_RS04320	913119..913475	-	357	WP_001030484.1	type II toxin-antitoxin system HicB family antitoxin	-
CF59_RS04325	913468..913746	-	279	WP_000598760.1	type II toxin-antitoxin system HicA family toxin	-
CF59_RS04330	913851..915563	-	1713	Protein_790	flagellar biosynthesis protein FlhA	-
CF59_RS04335	915535..916320	+	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
CF59_RS04340	916391..917446	+	1056	WP_001226166.1	DNA polymerase IV	-
CF59_RS04345	917498..917791	+	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
CF59_RS04350	917794..918192	+	399	WP_025210215.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
CF59_RS04355	918202..918654	+	453	WP_064734312.1	GNAT family N-acetyltransferase	-
CF59_RS04360	918691..919080	+	390	Protein_796	peptide chain release factor H	-
CF59_RS04365	919137..920594	-	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
CF59_RS04370	920855..921313	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
CF59_RS04375	921405..922649	+	1245	WP_000189541.1	esterase FrsA	-
-	921909..921989	+	81	NuclAT_10	-	-
-	921909..921989	+	81	NuclAT_10	-	-
-	921909..921989	+	81	NuclAT_10	-	-
-	921909..921989	+	81	NuclAT_10	-	-
CF59_RS04380	922707..923108	+	402	WP_000174684.1	sigma factor-binding protein Crl	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15519.97 Da
• Isoelectric Point: 8.9027

>T46350 WP_025210215.1 NZ_CP007393:917794-918192 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGILPDIFGRYALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T46350 NZ_CP007393:917794-918192 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTATTTTGCCAGATATATTTGGTCGCTATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAGCCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGAAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT46350 WP_000554758.1 NZ_CP007393:917498-917791 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT46350 NZ_CP007393:917498-917791 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CAGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGTTTCCGTCCGAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: S1QAE3