Detailed information of TA system TA046327

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 2086056..2086427
• Replicon: chromosome
• Accession: NZ_CP007392
• Organism: Escherichia coli strain ST2747

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: A0A3K0WCG2
• Locus tag: CF58_RS10060
• Protein ID: WP_032159264.1
• Coordinates: 2086233..2086427 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	2086056..2086234 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CF58_RS10035	2082011..2083384	+	1374	WP_001599773.1	ATP-dependent RNA helicase DbpA	-
CF58_RS10040	2083513..2084448	-	936	WP_001157407.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
CF58_RS25465	2084500..2085735	-	1236	WP_001516465.1	site-specific integrase	-
CF58_RS10050	2085737..2085952	-	216	WP_000079604.1	excisionase XisR	-
CF58_RS10055	2086052..2086240	-	189	WP_001605349.1	DUF1187 family protein	-
-	2086056..2086234	+	179	NuclAT_0	-	Antitoxin
-	2086056..2086234	+	179	NuclAT_0	-	Antitoxin
-	2086056..2086234	+	179	NuclAT_0	-	Antitoxin
-	2086056..2086234	+	179	NuclAT_0	-	Antitoxin
CF58_RS10060	2086233..2086427	-	195	WP_032159264.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
CF58_RS10065	2086491..2087543	-	1053	WP_001004422.1	recombinase RecT	-
CF58_RS10070	2087555..2090626	-	3072	WP_001605350.1	exodeoxyribonuclease VIII	-
CF58_RS10075	2090728..2091003	-	276	WP_001344816.1	hypothetical protein	-
CF58_RS10080	2091078..2091248	-	171	WP_001352098.1	conserved protein, Rac prophage	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2084500..2246945	162445

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7063.03 Da
• Isoelectric Point: 9.2886

>T46327 WP_032159264.1 NZ_CP007392:c2086427-2086233 [Escherichia coli]
MRYEKVKPCPFCGCLSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T46327 NZ_CP007392:c2086427-2086233 [Escherichia coli]
ATGCGGTATGAAAAAGTCAAACCATGCCCTTTTTGTGGTTGTCTATCAGTAACGGTAAAAGCCATTTCAGGATATTACCG
CGCGAAGTGTAACGGATGCGAATCCCGAACCGGCTATGGTGGAAGTGAAAAAGAAGCACTCGAACGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT46327 NZ_CP007392:2086056-2086234 [Escherichia coli]
GACGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAACCGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTGGTTAATTTTGTTTTTGAGTACCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A3K0WCG2

Antitoxin