Detailed information of TA system TA046320

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 909956..910650
• Replicon: chromosome
• Accession: NZ_CP007392
• Organism: Escherichia coli strain ST2747

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: -
• Locus tag: CF58_RS04315
• Protein ID: WP_025210215.1
• Coordinates: 910252..910650 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: CF58_RS04310
• Protein ID: WP_000554758.1
• Coordinates: 909956..910249 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CF58_RS04285	905577..905933	-	357	WP_001030484.1	type II toxin-antitoxin system HicB family antitoxin	-
CF58_RS04290	905926..906204	-	279	WP_000598760.1	type II toxin-antitoxin system HicA family toxin	-
CF58_RS04295	906309..908021	-	1713	Protein_792	flagellar biosynthesis protein FlhA	-
CF58_RS04300	907993..908778	+	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
CF58_RS04305	908849..909904	+	1056	WP_001226166.1	DNA polymerase IV	-
CF58_RS04310	909956..910249	+	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
CF58_RS04315	910252..910650	+	399	WP_025210215.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
CF58_RS04320	910660..911112	+	453	WP_064734312.1	GNAT family N-acetyltransferase	-
CF58_RS04325	911149..911538	+	390	Protein_798	peptide chain release factor H	-
CF58_RS04330	911595..913052	-	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
CF58_RS04335	913313..913771	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
CF58_RS04340	913863..915107	+	1245	WP_000189541.1	esterase FrsA	-
-	914367..914447	+	81	NuclAT_10	-	-
-	914367..914447	+	81	NuclAT_10	-	-
-	914367..914447	+	81	NuclAT_10	-	-
-	914367..914447	+	81	NuclAT_10	-	-
CF58_RS04345	915165..915566	+	402	WP_000174684.1	sigma factor-binding protein Crl	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15519.97 Da
• Isoelectric Point: 8.9027

>T46320 WP_025210215.1 NZ_CP007392:910252-910650 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGILPDIFGRYALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T46320 NZ_CP007392:910252-910650 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTATTTTGCCAGATATATTTGGTCGCTATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAGCCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGAAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT46320 WP_000554758.1 NZ_CP007392:909956-910249 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT46320 NZ_CP007392:909956-910249 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CAGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGTTTCCGTCCGAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: S1QAE3