Detailed information of TA system TA046313

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sib/-
• Location: 4114406..4114832
• Replicon: chromosome
• Accession: NZ_CP007391
• Organism: Escherichia coli strain ST540

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: CF61_RS20625
• Protein ID: WP_001312861.1
• Coordinates: 4114674..4114832 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sib
Locus tag	-
Coordinates	4114406..4114630 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CF61_RS20580	4109481..4109687	+	207	WP_001774176.1	hypothetical protein	-
CF61_RS28465	4110098..4110304	+	207	WP_000275853.1	hypothetical protein	-
CF61_RS20595	4110330..4110869	+	540	WP_000290839.1	single-stranded DNA-binding protein	-
CF61_RS20600	4110932..4111165	+	234	WP_000005990.1	DUF905 family protein	-
CF61_RS20605	4111231..4113189	+	1959	WP_025269850.1	ParB/RepB/Spo0J family partition protein	-
CF61_RS20610	4113244..4113678	+	435	WP_001470779.1	conjugation system SOS inhibitor PsiB	-
CF61_RS20615	4113675..4114394	+	720	WP_001276232.1	plasmid SOS inhibition protein A	-
CF61_RS28265	4114406..4114594	-	189	WP_001299721.1	hypothetical protein	-
-	4114406..4114630	+	225	NuclAT_0	-	-
-	4114406..4114630	+	225	NuclAT_0	-	-
-	4114406..4114630	+	225	NuclAT_0	-	-
-	4114406..4114630	+	225	NuclAT_0	-	-
CF61_RS20625	4114674..4114832	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
CF61_RS28470	4115523..4115729	+	207	WP_000547974.1	hypothetical protein	-
CF61_RS20640	4115754..4116041	+	288	WP_000107535.1	hypothetical protein	-
CF61_RS20645	4116160..4116981	+	822	WP_025269852.1	DUF945 domain-containing protein	-
CF61_RS20650	4117278..4117787	-	510	WP_025269853.1	transglycosylase SLT domain-containing protein	-
CF61_RS20655	4118202..4118585	+	384	WP_025269854.1	relaxosome protein TraM	-
CF61_RS20660	4118776..4119462	+	687	WP_025269855.1	PAS domain-containing protein	-
CF61_RS20665	4119556..4119783	+	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4073654..4117787	44133

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T46313 WP_001312861.1 NZ_CP007391:4114674-4114832 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T46313 NZ_CP007391:4114674-4114832 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT46313 NZ_CP007391:c4114630-4114406 [Escherichia coli]
ATGTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCTTTCTGCCACACA
ACACGGCAACAAACCACCTTCACGTCATGAGGCAGAAAGCCTCAAGCGCCGGGCACATCATAGCCCATATACCTGCACGC
TGACCACACTCACTTTCCCTGAAAATAATCCGCTCATTCAGACCGTTCACGGGAAATCCGTGTGA

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin