Detailed information of TA system TA046310

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3708941..3709635
• Replicon: chromosome
• Accession: NZ_CP007391
• Organism: Escherichia coli strain ST540

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: CF61_RS18570
• Protein ID: WP_001263493.1
• Coordinates: 3708941..3709339 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: CF61_RS18575
• Protein ID: WP_000554757.1
• Coordinates: 3709342..3709635 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CF61_RS18540	3703941..3705185	-	1245	WP_000189541.1	esterase FrsA	-
-	3704601..3704681	-	81	NuclAT_11	-	-
-	3704601..3704681	-	81	NuclAT_11	-	-
-	3704601..3704681	-	81	NuclAT_11	-	-
-	3704601..3704681	-	81	NuclAT_11	-	-
CF61_RS18545	3705277..3705735	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
CF61_RS18550	3705996..3707453	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
CF61_RS18555	3707510..3708031	-	522	Protein_3527	peptide chain release factor H	-
CF61_RS18560	3708030..3708233	-	204	Protein_3528	RNA ligase RtcB family protein	-
CF61_RS18565	3708479..3708931	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
CF61_RS18570	3708941..3709339	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
CF61_RS18575	3709342..3709635	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
CF61_RS18580	3709687..3710741	-	1055	Protein_3532	DNA polymerase IV	-
CF61_RS18585	3710812..3711735	-	924	WP_025269817.1	putative lateral flagellar export/assembly protein LafU	-
CF61_RS18590	3711738..3712601	-	864	WP_001169518.1	flagellar motor stator protein MotA	-
CF61_RS18595	3712614..3713330	-	717	WP_000938723.1	FliA/WhiG family RNA polymerase sigma factor	-
CF61_RS18600	3713350..3713817	-	468	WP_000725257.1	flagellar basal body-associated FliL family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T46310 WP_001263493.1 NZ_CP007391:c3709339-3708941 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T46310 NZ_CP007391:c3709339-3708941 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT46310 WP_000554757.1 NZ_CP007391:c3709635-3709342 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT46310 NZ_CP007391:c3709635-3709342 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6