Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1900266..1900768 Replicon chromosome
Accession NZ_CP007391
Organism Escherichia coli strain ST540

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag CF61_RS09385 Protein ID WP_000767829.1
Coordinates 1900514..1900768 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag CF61_RS09380 Protein ID WP_001259255.1
Coordinates 1900266..1900517 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CF61_RS09360 1895443..1896510 - 1068 WP_000080090.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
CF61_RS09365 1896510..1897580 - 1071 WP_000108933.1 histidinol-phosphate transaminase -
CF61_RS09370 1897577..1898881 - 1305 WP_001446927.1 histidinol dehydrogenase -
CF61_RS09375 1898887..1899786 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
CF61_RS25225 1899932..1899982 - 51 WP_001364200.1 his operon leader peptide -
CF61_RS09380 1900266..1900517 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
CF61_RS09385 1900514..1900768 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
CF61_RS09390 1900851..1901675 + 825 WP_000754737.1 SDR family oxidoreductase -
CF61_RS09395 1901721..1902650 + 930 WP_000803366.1 LysR family transcriptional regulator -
CF61_RS27560 1902865..1902927 + 63 WP_010723108.1 membrane protein YoeI -
CF61_RS09400 1902917..1904275 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
CF61_RS09405 1904454..1905512 + 1059 WP_000492321.1 transport protein YeeE -
CF61_RS09410 1905526..1905753 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T46299 WP_000767829.1 NZ_CP007391:1900514-1900768 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T46299 NZ_CP007391:1900514-1900768 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT46299 WP_001259255.1 NZ_CP007391:1900266-1900517 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT46299 NZ_CP007391:1900266-1900517 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References