Detailed information of TA system TA046290

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sib/-
• Location: 4067455..4067881
• Replicon: chromosome
• Accession: NZ_CP007390
• Organism: Escherichia coli strain ST540

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: CF57_RS20405
• Protein ID: WP_001312861.1
• Coordinates: 4067723..4067881 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sib
Locus tag	-
Coordinates	4067455..4067679 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CF57_RS20360	4062530..4062736	+	207	WP_001774176.1	hypothetical protein	-
CF57_RS26730	4062655..4062906	+	252	WP_071579736.1	hypothetical protein	-
CF57_RS27880	4063147..4063353	+	207	WP_000275853.1	hypothetical protein	-
CF57_RS20375	4063379..4063918	+	540	WP_000290839.1	single-stranded DNA-binding protein	-
CF57_RS20380	4063981..4064214	+	234	WP_000005990.1	DUF905 family protein	-
CF57_RS20385	4064280..4066238	+	1959	WP_025269850.1	ParB/RepB/Spo0J family partition protein	-
CF57_RS20390	4066293..4066727	+	435	WP_001470779.1	conjugation system SOS inhibitor PsiB	-
CF57_RS20395	4066724..4067443	+	720	WP_001276232.1	plasmid SOS inhibition protein A	-
CF57_RS27700	4067455..4067643	-	189	WP_001299721.1	hypothetical protein	-
-	4067455..4067679	+	225	NuclAT_0	-	-
-	4067455..4067679	+	225	NuclAT_0	-	-
-	4067455..4067679	+	225	NuclAT_0	-	-
-	4067455..4067679	+	225	NuclAT_0	-	-
CF57_RS20405	4067723..4067881	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
CF57_RS27885	4068176..4068331	+	156	WP_001380190.1	hypothetical protein	-
CF57_RS27890	4068572..4068778	+	207	WP_000547974.1	hypothetical protein	-
CF57_RS20420	4068803..4069090	+	288	WP_000107535.1	hypothetical protein	-
CF57_RS20425	4069209..4070030	+	822	WP_025269852.1	DUF945 domain-containing protein	-
CF57_RS20430	4070327..4070836	-	510	WP_025269853.1	transglycosylase SLT domain-containing protein	-
CF57_RS20435	4071251..4071634	+	384	WP_025269854.1	relaxosome protein TraM	-
CF57_RS20440	4071825..4072511	+	687	WP_025269855.1	PAS domain-containing protein	-
CF57_RS20445	4072605..4072832	+	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4004388..4070836	66448

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T46290 WP_001312861.1 NZ_CP007390:4067723-4067881 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T46290 NZ_CP007390:4067723-4067881 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT46290 NZ_CP007390:c4067679-4067455 [Escherichia coli]
ATGTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCTTTCTGCCACACA
ACACGGCAACAAACCACCTTCACGTCATGAGGCAGAAAGCCTCAAGCGCCGGGCACATCATAGCCCATATACCTGCACGC
TGACCACACTCACTTTCCCTGAAAATAATCCGCTCATTCAGACCGTTCACGGGAAATCCGTGTGA

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin