Detailed information of TA system TA046268

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4312976..4313670
• Replicon: chromosome
• Accession: NZ_CP007275
• Organism: Escherichia coli strain O18

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1PJQ2
• Locus tag: NMECO18_RS21415
• Protein ID: WP_001263500.1
• Coordinates: 4313272..4313670 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: NMECO18_RS21410
• Protein ID: WP_000554758.1
• Coordinates: 4312976..4313269 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NMECO18_RS21390	4308512..4309009	+	498	WP_000006241.1	REP-associated tyrosine transposase RayT	-
NMECO18_RS26970	4309092..4309250	-	159	WP_014639450.1	hypothetical protein	-
NMECO18_RS21395	4309329..4311041	-	1713	Protein_4044	flagellar biosynthesis protein FlhA	-
NMECO18_RS21400	4311013..4311798	+	786	WP_000207544.1	putative lateral flagellar export/assembly protein LafU	-
NMECO18_RS21405	4311869..4312924	+	1056	WP_001226168.1	DNA polymerase IV	-
NMECO18_RS21410	4312976..4313269	+	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
NMECO18_RS21415	4313272..4313670	+	399	WP_001263500.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
NMECO18_RS21420	4313680..4314132	+	453	WP_001059895.1	GNAT family N-acetyltransferase	-
NMECO18_RS21425	4314322..4315461	+	1140	WP_000521577.1	RNA ligase RtcB family protein	-
NMECO18_RS21430	4315458..4316072	+	615	WP_000602123.1	peptide chain release factor H	-
NMECO18_RS21435	4316129..4317586	-	1458	WP_001292999.1	cytosol nonspecific dipeptidase	-
NMECO18_RS21440	4317847..4318305	+	459	WP_001291988.1	xanthine phosphoribosyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15413.85 Da
• Isoelectric Point: 8.9511

>T46268 WP_001263500.1 NZ_CP007275:4313272-4313670 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T46268 NZ_CP007275:4313272-4313670 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGAAGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGTGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGCAGCGCATTTGGTATATTGTCAGGGCGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT46268 WP_000554758.1 NZ_CP007275:4312976-4313269 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT46268 NZ_CP007275:4312976-4313269 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
TGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTTGAAGCATTAATGGACATGCTTG
CAGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCGAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1PJQ2

Antitoxin

• AlphaFold DB: S1QAE3