Detailed information of TA system TA046263

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 3436897..3437499
• Replicon: chromosome
• Accession: NZ_CP007275
• Organism: Escherichia coli strain O18

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: NMECO18_RS17210
• Protein ID: WP_000897302.1
• Coordinates: 3436897..3437208 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: NMECO18_RS17215
• Protein ID: WP_000356397.1
• Coordinates: 3437209..3437499 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NMECO18_RS17185	3432811..3433410	+	600	WP_001314324.1	glucose-1-phosphatase	-
NMECO18_RS17190	3433404..3434276	+	873	WP_000920762.1	virulence factor BrkB family protein	-
NMECO18_RS17195	3434273..3434710	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
NMECO18_RS17200	3434755..3435696	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
NMECO18_RS17205	3435760..3436668	-	909	WP_001331553.1	alpha/beta hydrolase	-
NMECO18_RS17210	3436897..3437208	+	312	WP_000897302.1	hypothetical protein	Toxin
NMECO18_RS17215	3437209..3437499	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
NMECO18_RS17225	3437858..3438136	+	279	WP_001296612.1	hypothetical protein	-
NMECO18_RS17230	3438532..3438750	+	219	WP_001314326.1	ribbon-helix-helix domain-containing protein	-
NMECO18_RS17235	3438966..3439895	-	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
NMECO18_RS17240	3439892..3440527	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
NMECO18_RS17245	3440524..3441426	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T46263 WP_000897302.1 NZ_CP007275:3436897-3437208 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T46263 NZ_CP007275:3436897-3437208 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT46263 WP_000356397.1 NZ_CP007275:3437209-3437499 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT46263 NZ_CP007275:3437209-3437499 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: S1P416