Detailed information of TA system TA046244

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 468329..468554
• Replicon: chromosome
• Accession: NZ_CP007275
• Organism: Escherichia coli strain O18

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: A0A8S7B7V6
• Locus tag: NMECO18_RS02565
• Protein ID: WP_000813256.1
• Coordinates: 468399..468554 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	468329..468387 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NMECO18_RS02530	463354..463596	+	243	WP_000747951.1	hypothetical protein	-
NMECO18_RS02535	463580..464005	+	426	WP_000693867.1	Rha family transcriptional regulator	-
NMECO18_RS02540	464077..465147	+	1071	WP_001262357.1	hypothetical protein	-
NMECO18_RS02545	465188..465610	+	423	WP_001151216.1	DUF977 family protein	-
NMECO18_RS02550	465802..466764	+	963	WP_014639476.1	hypothetical protein	-
NMECO18_RS02555	466780..467781	+	1002	WP_000354966.1	hypothetical protein	-
-	468329..468387	-	59	-	-	Antitoxin
NMECO18_RS02565	468399..468554	+	156	WP_000813256.1	type I toxin-antitoxin system toxin HokD	Toxin
NMECO18_RS02570	468722..469000	+	279	WP_011478175.1	hypothetical protein	-
NMECO18_RS02575	469002..470048	+	1047	WP_001265108.1	DUF968 domain-containing protein	-
NMECO18_RS02580	470061..470435	+	375	WP_000904114.1	RusA family crossover junction endodeoxyribonuclease	-
NMECO18_RS02585	470432..471253	+	822	WP_000762880.1	antitermination protein	-
NMECO18_RS02590	471478..471675	+	198	WP_000917749.1	hypothetical protein	-
NMECO18_RS02595	471826..472875	+	1050	WP_000935515.1	site-specific DNA-methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	452736..505276	52540

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5764.94 Da
• Isoelectric Point: 6.1531

>T46244 WP_000813256.1 NZ_CP007275:468399-468554 [Escherichia coli]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTVYEPEE

Nucleotide

• Length: 156 bp

>T46244 NZ_CP007275:468399-468554 [Escherichia coli]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGTTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT46244 NZ_CP007275:c468387-468329 [Escherichia coli]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTAGATTTGCCGTTCATAGATTGAGCCTCA

Structures

Antitoxin