Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 3530248..3530750 Replicon chromosome
Accession NZ_CP007265
Organism Escherichia coli strain ST540

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag BU34_RS17600 Protein ID WP_000767829.1
Coordinates 3530496..3530750 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag BU34_RS17595 Protein ID WP_001259255.1
Coordinates 3530248..3530499 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BU34_RS17575 3525425..3526492 - 1068 WP_000080090.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
BU34_RS17580 3526492..3527562 - 1071 WP_000108933.1 histidinol-phosphate transaminase -
BU34_RS17585 3527559..3528863 - 1305 WP_001446927.1 histidinol dehydrogenase -
BU34_RS17590 3528869..3529768 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
BU34_RS25195 3529914..3529964 - 51 WP_001364200.1 his operon leader peptide -
BU34_RS17595 3530248..3530499 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
BU34_RS17600 3530496..3530750 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
BU34_RS17605 3530833..3531657 + 825 WP_000754737.1 SDR family oxidoreductase -
BU34_RS17610 3531703..3532632 + 930 WP_000803366.1 LysR family transcriptional regulator -
BU34_RS26715 3532847..3532909 + 63 WP_010723108.1 membrane protein YoeI -
BU34_RS17615 3532899..3534257 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
BU34_RS17620 3534436..3535494 + 1059 WP_000492321.1 transport protein YeeE -
BU34_RS17625 3535508..3535735 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T46229 WP_000767829.1 NZ_CP007265:3530496-3530750 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T46229 NZ_CP007265:3530496-3530750 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT46229 WP_001259255.1 NZ_CP007265:3530248-3530499 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT46229 NZ_CP007265:3530248-3530499 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References