Detailed information of TA system TA046220

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sib/-
• Location: 1493645..1494071
• Replicon: chromosome
• Accession: NZ_CP007265
• Organism: Escherichia coli strain ST540

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: BU34_RS07575
• Protein ID: WP_001312861.1
• Coordinates: 1493913..1494071 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sib
Locus tag	-
Coordinates	1493645..1493869 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BU34_RS07530	1488720..1488926	+	207	WP_001774176.1	hypothetical protein	-
BU34_RS27355	1489337..1489543	+	207	WP_000275853.1	hypothetical protein	-
BU34_RS07545	1489569..1490108	+	540	WP_000290839.1	single-stranded DNA-binding protein	-
BU34_RS07550	1490171..1490404	+	234	WP_000005990.1	DUF905 family protein	-
BU34_RS07555	1490470..1492428	+	1959	WP_025269850.1	ParB/RepB/Spo0J family partition protein	-
BU34_RS07560	1492483..1492917	+	435	WP_001470779.1	conjugation system SOS inhibitor PsiB	-
BU34_RS07565	1492914..1493633	+	720	WP_001276232.1	plasmid SOS inhibition protein A	-
BU34_RS27190	1493645..1493833	-	189	WP_001299721.1	hypothetical protein	-
-	1493645..1493869	+	225	NuclAT_0	-	-
-	1493645..1493869	+	225	NuclAT_0	-	-
-	1493645..1493869	+	225	NuclAT_0	-	-
-	1493645..1493869	+	225	NuclAT_0	-	-
BU34_RS07575	1493913..1494071	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
BU34_RS27360	1494762..1494968	+	207	WP_000547974.1	hypothetical protein	-
BU34_RS07590	1494993..1495280	+	288	WP_000107535.1	hypothetical protein	-
BU34_RS07595	1495399..1496220	+	822	WP_025269852.1	DUF945 domain-containing protein	-
BU34_RS07600	1496517..1497026	-	510	WP_025269853.1	transglycosylase SLT domain-containing protein	-
BU34_RS07605	1497441..1497824	+	384	WP_025269854.1	relaxosome protein TraM	-
BU34_RS07610	1498015..1498701	+	687	WP_025269855.1	PAS domain-containing protein	-
BU34_RS07615	1498795..1499022	+	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1460269..1497026	36757

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T46220 WP_001312861.1 NZ_CP007265:1493913-1494071 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T46220 NZ_CP007265:1493913-1494071 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT46220 NZ_CP007265:c1493869-1493645 [Escherichia coli]
ATGTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCTTTCTGCCACACA
ACACGGCAACAAACCACCTTCACGTCATGAGGCAGAAAGCCTCAAGCGCCGGGCACATCATAGCCCATATACCTGCACGC
TGACCACACTCACTTTCCCTGAAAATAATCCGCTCATTCAGACCGTTCACGGGAAATCCGTGTGA

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin