Detailed information of TA system TA046217

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 1036010..1036704
• Replicon: chromosome
• Accession: NZ_CP007265
• Organism: Escherichia coli strain ST540

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: BU34_RS05210
• Protein ID: WP_001263493.1
• Coordinates: 1036010..1036408 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: BU34_RS05215
• Protein ID: WP_000554757.1
• Coordinates: 1036411..1036704 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BU34_RS05180	1031010..1032254	-	1245	WP_000189541.1	esterase FrsA	-
-	1031670..1031750	-	81	NuclAT_11	-	-
-	1031670..1031750	-	81	NuclAT_11	-	-
-	1031670..1031750	-	81	NuclAT_11	-	-
-	1031670..1031750	-	81	NuclAT_11	-	-
BU34_RS05185	1032346..1032804	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
BU34_RS05190	1033065..1034522	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
BU34_RS05195	1034579..1035100	-	522	Protein_986	peptide chain release factor H	-
BU34_RS05200	1035099..1035302	-	204	Protein_987	RNA ligase RtcB family protein	-
BU34_RS05205	1035548..1036000	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
BU34_RS05210	1036010..1036408	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
BU34_RS05215	1036411..1036704	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
BU34_RS05220	1036756..1037811	-	1056	WP_001226164.1	DNA polymerase IV	-
BU34_RS05225	1037882..1038805	-	924	WP_025269817.1	putative lateral flagellar export/assembly protein LafU	-
BU34_RS05230	1038808..1039671	-	864	WP_001169518.1	flagellar motor stator protein MotA	-
BU34_RS05235	1039684..1040400	-	717	WP_000938723.1	FliA/WhiG family RNA polymerase sigma factor	-
BU34_RS05240	1040420..1040887	-	468	WP_000725257.1	flagellar basal body-associated FliL family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T46217 WP_001263493.1 NZ_CP007265:c1036408-1036010 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T46217 NZ_CP007265:c1036408-1036010 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT46217 WP_000554757.1 NZ_CP007265:c1036704-1036411 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT46217 NZ_CP007265:c1036704-1036411 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6