Detailed information of TA system TA046186

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4583754..4584496
• Replicon: chromosome
• Accession: NZ_CP007215
• Organism: Kosakonia sacchari SP1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A1G4Z0Q6
• Locus tag: C813_RS44625
• Protein ID: WP_017459412.1
• Coordinates: 4584185..4584496 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C813_RS44620
• Protein ID: WP_017459413.1
• Coordinates: 4583754..4584188 (-)
• Length: 145 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C813_RS44605	4579325..4580467	-	1143	WP_017459416.1	slipin family protein	-
C813_RS44610	4580938..4582524	+	1587	WP_017459415.1	RNA repair transcriptional activator RtcR	-
C813_RS44615	4582776..4583717	+	942	WP_017459414.1	ketopantoate/pantoate/pantothenate transporter PanS	-
C813_RS44620	4583754..4584188	-	435	WP_017459413.1	helix-turn-helix domain-containing protein	Antitoxin
C813_RS44625	4584185..4584496	-	312	WP_017459412.1	type II toxin-antitoxin system HigB family toxin	Toxin
C813_RS44630	4584744..4585016	+	273	WP_017459411.1	DUF3811 domain-containing protein	-
C813_RS44635	4585017..4585880	-	864	WP_017459410.1	23S rRNA pseudouridine(2604) synthase RluF	-
C813_RS44640	4586017..4587648	-	1632	WP_017459409.1	Na/Pi cotransporter family protein	-
C813_RS44645	4587820..4588296	-	477	WP_051487234.1	hypothetical protein	-
C813_RS44650	4588271..4589047	-	777	WP_017459407.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (81-135)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12416.30 Da
• Isoelectric Point: 9.2998

>T46186 WP_017459412.1 NZ_CP007215:c4584496-4584185 [Kosakonia sacchari SP1]
MHVISRRPFDNAAKDFPNHTLALDAVYKILKSTVYKNPDELREEFPSLDKMKYRDKWWVINVGGNHLRVLFFADFELAKI
FVKHIVPHDEYDKLIRHYRETKE

Nucleotide

• Length: 312 bp

>T46186 NZ_CP007215:c4584496-4584185 [Kosakonia sacchari SP1]
ATGCACGTTATATCAAGGAGGCCGTTCGATAACGCGGCTAAAGATTTTCCAAACCATACGCTGGCGCTGGACGCGGTTTA
CAAAATCCTGAAAAGCACTGTTTACAAGAATCCAGACGAGTTGAGAGAAGAGTTCCCAAGCCTGGATAAGATGAAATACA
GGGATAAATGGTGGGTCATCAATGTCGGTGGCAACCATTTGAGAGTGTTATTTTTCGCAGATTTTGAATTAGCAAAGATA
TTTGTCAAACATATCGTGCCCCATGACGAATATGACAAGCTGATAAGACATTACCGGGAGACCAAAGAATGA

Antitoxin

Protein

• Length: 145 a.a.
• Molecular weight: 16013.14 Da
• Isoelectric Point: 4.9794

>AT46186 WP_017459413.1 NZ_CP007215:c4584188-4583754 [Kosakonia sacchari SP1]
MIEDAIKAADELVKLVPFLGNNPDKEDYEHALEMVEQLLTHVPDSSLVALLTAQIEHYENNDPELAAFNARIAALPRGVA
ALRVLMDQHGLNQSSFRDEIGQRSLVSRILNGERNLTVDHIRALAKRFNVSTDVFIEPAHHIAG

Nucleotide

• Length: 435 bp

>AT46186 NZ_CP007215:c4584188-4583754 [Kosakonia sacchari SP1]
ATGATTGAGGATGCGATCAAGGCCGCTGACGAGCTGGTGAAACTGGTGCCTTTTTTGGGAAATAACCCCGACAAAGAGGA
TTATGAACACGCGCTGGAGATGGTTGAACAGTTGCTCACGCATGTGCCGGATAGCTCGCTGGTTGCGCTGCTTACCGCAC
AGATAGAGCACTATGAAAATAACGATCCGGAACTGGCGGCGTTTAACGCGCGTATCGCGGCACTGCCACGCGGCGTTGCC
GCCTTACGGGTTTTGATGGATCAGCACGGATTAAATCAGTCCTCGTTTCGGGATGAAATCGGCCAGCGCTCCCTGGTGAG
CCGCATTCTTAATGGCGAACGCAATCTCACTGTCGACCATATCCGGGCGCTGGCGAAACGCTTTAATGTGTCGACCGACG
TGTTTATTGAGCCAGCCCATCACATCGCGGGGTGA

Structures

Toxin

• AlphaFold DB: A0A1G4Z0Q6