Detailed information of TA system TA046184

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4217417..4218147
• Replicon: chromosome
• Accession: NZ_CP007215
• Organism: Kosakonia sacchari SP1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A1G4Y5D2
• Locus tag: C813_RS42910
• Protein ID: WP_017457772.1
• Coordinates: 4217417..4217734 (+)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C813_RS42915
• Protein ID: WP_017457771.1
• Coordinates: 4217731..4218147 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C813_RS42900	4214771..4216135	+	1365	WP_017457774.1	efflux transporter outer membrane subunit	-
C813_RS42905	4216177..4217337	+	1161	WP_017457773.1	multidrug effflux MFS transporter	-
C813_RS42910	4217417..4217734	+	318	WP_017457772.1	type II toxin-antitoxin system HigB family toxin	Toxin
C813_RS42915	4217731..4218147	+	417	WP_017457771.1	helix-turn-helix domain-containing protein	Antitoxin
C813_RS42920	4218144..4219037	-	894	WP_017457770.1	MurR/RpiR family transcriptional regulator	-
C813_RS42925	4219047..4219994	-	948	WP_017457769.1	iron-siderophore ABC transporter substrate-binding protein	-
C813_RS42930	4220011..4221063	-	1053	WP_017457768.1	iron ABC transporter permease	-
C813_RS42935	4221060..4222085	-	1026	WP_017457767.1	iron ABC transporter permease	-
C813_RS42940	4222082..4222909	-	828	WP_017457766.1	ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (83-133)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12508.70 Da
• Isoelectric Point: 10.3626

>T46184 WP_017457772.1 NZ_CP007215:4217417-4217734 [Kosakonia sacchari SP1]
MHLISLKAIQEAVLRFPQHKVALLTLAKTIEKAHCPTPEDLKKLYPTLDNFKYLSKHYIIDIANNNLRVVALIFFESQKF
YVRHIFTHKEYDRFTEQHRTKGKKK

Nucleotide

• Length: 318 bp

>T46184 NZ_CP007215:4217417-4217734 [Kosakonia sacchari SP1]
ATGCATTTAATATCATTGAAGGCGATTCAGGAAGCAGTCTTACGCTTCCCTCAACACAAAGTTGCGCTACTCACTCTGGC
GAAAACCATCGAAAAAGCGCACTGCCCAACGCCAGAGGATCTCAAGAAGTTGTATCCCACACTGGATAACTTCAAATATC
TGAGTAAGCATTACATTATTGATATTGCGAATAATAATTTAAGGGTTGTGGCACTGATCTTTTTCGAAAGCCAAAAGTTT
TATGTGCGCCATATCTTCACACATAAGGAATACGACCGTTTTACCGAACAGCATCGTACTAAGGGGAAGAAAAAATGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15320.72 Da
• Isoelectric Point: 4.6233

>AT46184 WP_017457771.1 NZ_CP007215:4217731-4218147 [Kosakonia sacchari SP1]
MIVADALKATHALIAAVPLLGEHPTEKDYQDALELVEQLLIDNPDSPLVDIVCAKISAYENNQPEIIALREEMAAMPTGL
AVLRTLMDQHQLTTSDFQEEIGSKSMVSRVLNGERQLNLNHIKKLAKRFDVSPLMFIS

Nucleotide

• Length: 417 bp

>AT46184 NZ_CP007215:4217731-4218147 [Kosakonia sacchari SP1]
ATGATCGTCGCTGATGCATTAAAAGCCACCCATGCGCTCATCGCTGCGGTCCCGCTGCTGGGAGAACATCCGACGGAGAA
AGACTATCAGGATGCGCTGGAACTGGTAGAACAGCTGTTAATAGACAATCCCGATAGCCCGCTGGTAGATATCGTTTGTG
CGAAAATTAGTGCTTACGAAAATAACCAACCGGAAATTATCGCCTTGCGTGAAGAGATGGCGGCGATGCCGACAGGACTT
GCGGTGCTCAGAACACTGATGGATCAGCACCAACTGACGACGTCTGATTTTCAGGAGGAAATTGGCAGCAAATCGATGGT
ATCGAGAGTGCTCAACGGCGAGCGCCAGCTTAACCTTAACCATATTAAGAAGTTAGCCAAACGTTTTGACGTATCACCGC
TGATGTTTATTTCTTAA

Structures

Toxin

• AlphaFold DB: A0A1G4Y5D2