Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 1061729..1062378 Replicon chromosome
Accession NZ_CP007215
Organism Kosakonia sacchari SP1

Toxin (Protein)


Gene name higB Uniprot ID A0A1G4YX45
Locus tag C813_RS28030 Protein ID WP_017459286.1
Coordinates 1061729..1062091 (+) Length 121 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A1G4YWY7
Locus tag C813_RS28035 Protein ID WP_017459287.1
Coordinates 1062079..1062378 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C813_RS28005 1057075..1058079 - 1005 WP_017459281.1 LacI family transcriptional regulator -
C813_RS28010 1058119..1059978 - 1860 WP_017459282.1 beta-glucoside-specific PTS transporter subunit IIABC -
C813_RS28015 1060210..1060524 + 315 WP_017459283.1 antibiotic biosynthesis monooxygenase -
C813_RS28020 1060526..1060918 + 393 WP_017459284.1 amino acid-binding protein -
C813_RS28025 1060915..1061622 + 708 WP_017459285.1 winged helix-turn-helix domain-containing protein -
C813_RS28030 1061729..1062091 + 363 WP_017459286.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
C813_RS28035 1062079..1062378 + 300 WP_017459287.1 helix-turn-helix domain-containing protein Antitoxin
C813_RS28040 1062653..1063420 + 768 WP_017459288.1 isocitrate lyase/phosphoenolpyruvate mutase family protein -
C813_RS28045 1063404..1064456 + 1053 WP_017459289.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
C813_RS28050 1064531..1066360 - 1830 WP_017459290.1 glycoside hydrolase family 15 protein -
C813_RS28055 1066431..1067324 - 894 WP_017459291.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-107)

Antitoxin

Cro/C1-type helix-turn-helix domain

(32-84)


Sequences

Toxin        


Download         Length: 121 a.a.        Molecular weight: 14086.20 Da        Isoelectric Point: 8.9002

>T46170 WP_017459286.1 NZ_CP007215:1061729-1062091 [Kosakonia sacchari SP1]
MWQVITTERFDVWFSIQPELLQDEVLAVFRILSEFGPQLGRPYVDTIKGSTYSNMKELRIQYAGSPVRAFFAFDSTRRAI
VLCAADKTGVNEKRFYKDMIKIADAEFRNHMRNKELTWQP

Download         Length: 363 bp

>T46170 NZ_CP007215:1061729-1062091 [Kosakonia sacchari SP1]
ATGTGGCAAGTGATTACGACGGAGCGTTTTGACGTGTGGTTTAGCATCCAGCCAGAACTATTGCAGGATGAGGTTTTAGC
GGTTTTCAGGATCCTCAGTGAGTTTGGCCCGCAGCTTGGGCGTCCTTATGTCGATACCATAAAAGGATCAACCTATAGCA
ATATGAAAGAGTTGAGGATCCAGTATGCAGGTTCTCCCGTACGCGCCTTTTTTGCGTTCGACTCTACGCGGCGGGCTATT
GTACTGTGCGCCGCAGATAAAACAGGCGTTAACGAGAAACGGTTTTATAAAGACATGATAAAAATTGCCGACGCAGAGTT
CAGAAATCATATGCGAAATAAGGAGTTAACGTGGCAACCTTAG

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10989.63 Da        Isoelectric Point: 7.0165

>AT46170 WP_017459287.1 NZ_CP007215:1062079-1062378 [Kosakonia sacchari SP1]
VATLDELLAKRSPESQARIEERVDELRRNIVLSQLREELNISQTELAGVMGVKQPTLAKIEQPGNDPRLSTLKRYVSALG
GELSIDITLPTGKRVAFHI

Download         Length: 300 bp

>AT46170 NZ_CP007215:1062079-1062378 [Kosakonia sacchari SP1]
GTGGCAACCTTAGATGAACTTTTAGCGAAACGCAGCCCTGAAAGTCAGGCGCGAATTGAAGAGCGTGTTGATGAGCTGCG
CCGCAACATTGTATTGAGCCAGCTACGTGAAGAATTGAATATCTCCCAGACCGAGCTTGCCGGAGTCATGGGCGTTAAAC
AGCCGACACTGGCGAAGATAGAGCAGCCTGGCAACGATCCCCGTCTCTCTACTCTCAAGCGTTATGTCTCTGCGTTAGGT
GGCGAGTTAAGCATTGATATCACATTGCCGACAGGCAAACGCGTTGCATTTCATATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1G4YX45

Antitoxin

Source ID Structure
AlphaFold DB A0A1G4YWY7

References