Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/Txe-RelB
Location 2579008..2579525 Replicon chromosome
Accession NZ_CP007176
Organism Staphylococcus aureus USA300-ISMMS1

Toxin (Protein)


Gene name yoeB Uniprot ID T1YC79
Locus tag AZ30_RS13320 Protein ID WP_000113262.1
Coordinates 2579008..2579274 (-) Length 89 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID A0A5A8VWS3
Locus tag AZ30_RS13325 Protein ID WP_000584502.1
Coordinates 2579274..2579525 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AZ30_RS13295 2574434..2574970 - 537 WP_000169317.1 GNAT family N-acetyltransferase -
AZ30_RS13300 2575203..2576027 - 825 WP_000572040.1 formate/nitrite transporter family protein -
AZ30_RS13305 2576244..2576426 - 183 WP_000230294.1 hypothetical protein -
AZ30_RS13310 2576510..2576977 - 468 WP_000153344.1 SRPBCC domain-containing protein -
AZ30_RS13315 2577163..2578713 - 1551 WP_000727762.1 zinc ABC transporter substrate-binding lipoprotein AdcA -
AZ30_RS16130 2578902..2578973 - 72 Protein_2529 hypothetical protein -
AZ30_RS13320 2579008..2579274 - 267 WP_000113262.1 Txe/YoeB family addiction module toxin Toxin
AZ30_RS13325 2579274..2579525 - 252 WP_000584502.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
AZ30_RS13330 2579690..2579791 - 102 WP_001791744.1 hypothetical protein -
AZ30_RS13335 2579798..2580397 - 600 WP_000162813.1 DsbA family protein -
AZ30_RS13340 2580416..2580775 - 360 WP_000819847.1 DUF4467 domain-containing protein -
AZ30_RS13345 2581030..2582280 + 1251 WP_001012222.1 FemA/FemB family glycyltransferase FmhA -
AZ30_RS13350 2582377..2583108 - 732 WP_000615461.1 amino acid ABC transporter ATP-binding protein -
AZ30_RS13355 2583105..2583824 - 720 WP_000479576.1 amino acid ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-88)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-74)


Sequences

Toxin        


Download         Length: 89 a.a.        Molecular weight: 10437.95 Da        Isoelectric Point: 9.9143

>T46148 WP_000113262.1 NZ_CP007176:c2579274-2579008 [Staphylococcus aureus USA300-ISMMS1]
MARLNITFSPQAFEDYKYFQQNDKKMVKKINELLKSIDRNGALEGIGKPEKLKSNLTGYYSRRINHEHRLVYTVDDNHIK
IASCKYHY

Download         Length: 267 bp

>T46148 NZ_CP007176:c2579274-2579008 [Staphylococcus aureus USA300-ISMMS1]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACGATAAAAAAATGGT
GAAGAAGATTAATGAGTTACTTAAAAGTATTGACAGAAATGGTGCATTGGAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATCACGAACATAGATTGGTTTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9417.65 Da        Isoelectric Point: 4.4702

>AT46148 WP_000584502.1 NZ_CP007176:c2579525-2579274 [Staphylococcus aureus USA300-ISMMS1]
MIIKNYSYARQNLKALMTKVNDDSVMVTVTSTDDKNVVIMSESDYNSMMETLYLQQNPNNAEHLAQSIADLERGKTITKD
IDV

Download         Length: 252 bp

>AT46148 NZ_CP007176:c2579525-2579274 [Staphylococcus aureus USA300-ISMMS1]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGTTATGGT
AACTGTAACATCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAACCCAAATAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGGAAAACTATAACGAAAGAT
ATAGATGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6L8G
PDB 6L8F
PDB 7CUA
PDB 6L8E

Antitoxin

Source ID Structure
AlphaFold DB A0A5A8VWS3

References